Gajjela, Swetha
NEW VALIDATED METHOD DEVELOPMENT FOR THE ESTIMATION OF SULFAMETHOXAZOLE AND TRIMETHOPRIM IN BULK FORM BY VISIBLE SPECTROSCOPY - Vol.10(12) - M P Innovare Academic Sciences Pvt Ltd 2018 - 50-57p.
Objective:
To
develop
three
novel
, sensitive, simple
validated
visible
spectrophotometric methods
for
the quantitative estimation
of
sulfamethoxazole
(SMZ)
and
trimethoprim
(TMP)
in bulk form
.
Method
s:
Methods were based on coupling the d
iazotized
aromatic
primary amino group of the studied drugs with
o-
phenylenediamine
(OPD
) in
an acidic medium
. The first two methods have been proposed for estimation of SMZ
and rest
for
TMP
. The resulting products were m
easured by
spectrophotometric (m
ethod I, II
and
III) tools.
The methods we
re validated as per ICH guidelines
.
Result
s:
In
method
I, the absorbance was measured at 482 and
457 nm with linearity ranges of 4.0
-40.0 and 5.0
-45.0 μg
/ml
for
SMZ
. On the other
hand, method III was
devoted to estimate
TMP
spectrophotometrically
at 457 nm with
linearity range of 5
-
30 μg
/ml
. The
r
2
Conclusio
n:
The study concludes that
visible
spectrophotometric validation method
s can be very efficien
t and economically promising technique
for
the quantitative
analysis of S
MZ
and
TMP
in bulk form
. The statistical analysis of data indicates that the
developed methods were
reproducible
and specific.
It was found that there is a good agreement between the obtained results and those obtained by the reported method
s;
moreover
they
can be
used for
the routine estimations of
SMZ and TMP
in bulk form
PHARMACEUTICS
NEW VALIDATED METHOD DEVELOPMENT FOR THE ESTIMATION OF SULFAMETHOXAZOLE AND TRIMETHOPRIM IN BULK FORM BY VISIBLE SPECTROSCOPY - Vol.10(12) - M P Innovare Academic Sciences Pvt Ltd 2018 - 50-57p.
Objective:
To
develop
three
novel
, sensitive, simple
validated
visible
spectrophotometric methods
for
the quantitative estimation
of
sulfamethoxazole
(SMZ)
and
trimethoprim
(TMP)
in bulk form
.
Method
s:
Methods were based on coupling the d
iazotized
aromatic
primary amino group of the studied drugs with
o-
phenylenediamine
(OPD
) in
an acidic medium
. The first two methods have been proposed for estimation of SMZ
and rest
for
TMP
. The resulting products were m
easured by
spectrophotometric (m
ethod I, II
and
III) tools.
The methods we
re validated as per ICH guidelines
.
Result
s:
In
method
I, the absorbance was measured at 482 and
457 nm with linearity ranges of 4.0
-40.0 and 5.0
-45.0 μg
/ml
for
SMZ
. On the other
hand, method III was
devoted to estimate
TMP
spectrophotometrically
at 457 nm with
linearity range of 5
-
30 μg
/ml
. The
r
2
Conclusio
n:
The study concludes that
visible
spectrophotometric validation method
s can be very efficien
t and economically promising technique
for
the quantitative
analysis of S
MZ
and
TMP
in bulk form
. The statistical analysis of data indicates that the
developed methods were
reproducible
and specific.
It was found that there is a good agreement between the obtained results and those obtained by the reported method
s;
moreover
they
can be
used for
the routine estimations of
SMZ and TMP
in bulk form
PHARMACEUTICS