Tambe, V. S.
Bioactivity enhanced isolated carpaine from carica papaya leaves for platelet stimulating activity - Vol.83(4), July-Aug - Mumbai Indian Journal of Pharmaceutical Science 2021 - 723-731p.
Carica papaya leaves are used in folklore medicine for the treatment of different types of thrombocytopenia
associated with diseases and drugs. There are several scientific studies carried out on humans and animal
models to confirm the efficacy of papaya leaves extract for the treatment of thrombocytopenia. In the
present study, the alkaloid, carpaine extracted from papaya leaves was found to have platelet stimulating
activity. Papaya leaves powder was extracted by microwave with a mixture of methanol: glacial acetic acid:
water (180:2:1.6 v/v/v). The extract was treated with suitable solvent to obtain alkaloid fraction. From
the total alkaloids, carpaine was further separated by preparative thin layer chromatography, purified
and analyzed. Carpaine was complexed with beta-cyclodextrin and mixed with piperine. The complex
was administered in thrombocytopenia induced rats. The results showed that bioavailability enhanced
carpaine exhibits potent activity of increasing platelet count.
PHARMACEUTICS
Bioactivity enhanced isolated carpaine from carica papaya leaves for platelet stimulating activity - Vol.83(4), July-Aug - Mumbai Indian Journal of Pharmaceutical Science 2021 - 723-731p.
Carica papaya leaves are used in folklore medicine for the treatment of different types of thrombocytopenia
associated with diseases and drugs. There are several scientific studies carried out on humans and animal
models to confirm the efficacy of papaya leaves extract for the treatment of thrombocytopenia. In the
present study, the alkaloid, carpaine extracted from papaya leaves was found to have platelet stimulating
activity. Papaya leaves powder was extracted by microwave with a mixture of methanol: glacial acetic acid:
water (180:2:1.6 v/v/v). The extract was treated with suitable solvent to obtain alkaloid fraction. From
the total alkaloids, carpaine was further separated by preparative thin layer chromatography, purified
and analyzed. Carpaine was complexed with beta-cyclodextrin and mixed with piperine. The complex
was administered in thrombocytopenia induced rats. The results showed that bioavailability enhanced
carpaine exhibits potent activity of increasing platelet count.
PHARMACEUTICS