DEVELOPMENT AND VALIDATION OF ANALYTICAL SPECTROPHO TOMETRIC AND RP-HPLC METHODS FOR THE SIMULTANEOUS ESTIMATION OF HYDROQUI NONE, HYDROCORTISONE AND TRETINOIN TERNARY MIXTURE IN TOPICAL FORMULATION (Record no. 11098)
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| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20200207111915.0 |
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| fixed length control field | 200207b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 11991 |
| Author | Marwa, AlJamal K. |
| 245 ## - TITLE STATEMENT | |
| Title | DEVELOPMENT AND VALIDATION OF ANALYTICAL SPECTROPHO TOMETRIC AND RP-HPLC METHODS FOR THE SIMULTANEOUS ESTIMATION OF HYDROQUI NONE, HYDROCORTISONE AND TRETINOIN TERNARY MIXTURE IN TOPICAL FORMULATION |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.11(11) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | M P |
| Name of publisher, distributor, etc. | Innovare Academic Sciences Pvt Ltd |
| Year | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 10-16p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Objective: Development and validation of spectrophotometric an d RP-HPLC methods for the simultaneous determination of Hydroquinone (HQ), Hydrocortisone (HC) and Tretinoin (TRT) ternary combi nation in pharmaceutical preparation. Methods: The proposed spectrophotometric method was able to determine TRT directly from its absorption spectrum at 362 nm, however, HQ and HC from their first derivative spectra at 284 nm and 252 nm, respectively, without any separation step. The RP-HPLC method was developed using a C 18 Sunfire © waters column with a mobile phase composed of acet onitrile: phosphate buffer (adjusted to pH 6.1 using ortho-phosphoric acid) in the ratio of 30:70 %, v/v, respectively at a flow rate of 0.8 ml/min. Quantification was based on measurin g peak areas at 260 nm. Results: The spectrophotometric method was able to selective ly quantify each of HQ, HC and TRT in the ranges of 10-50 μg/ml, 2-10 μg/ml and 0.5-5 μg/ml, respectively. The RP-HPLC method was able to produc e well-resolved peaks after 3.0, 8.2 and 20.2 min, in the ranges of 2-10 μg/ml, 0.1-1 μg/ml and 0.05-2 μg/ml, for HQ, HC and TRT, respectively. The obtain ed A, D 1 or peak areas values plotted against the concentra tion of each of the three components showed linear response in the stated ranges. Both methods were validated in terms of linearity, LOD, LOQ, pre cision, accuracy and selectivity. Conclusion: Both developed proposed methods were applied for th e determination of the active ingredients in the ph armaceutical formulation and the common excipients did not interfere in the anal ysis. The RP-HPLC method proved to be more sensitiv e when compared to the applied spectrophotometric method. However, the applied spe ctrophotometric methods, considered as green analyt ical chemistry, is a simple, time-saving method that requires minimal use of a hazardous sol vent. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 11992 |
| Co-Author | Gazy, Azza A. |
| 773 0# - HOST ITEM ENTRY | |
| Title | International journal of pharmacy and pharmaceutical science |
| Place, publisher, and date of publication | Bhopal Innovare Academic Sciences Pvt Ltd |
| International Standard Serial Number | 2656-0097 |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://innovareacademics.in/journals/index.php/ijpps/article/view/35188/21008 |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Barcode | Date last seen | Price effective from | Koha item type |
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| School of Pharmacy | School of Pharmacy | Archieval Section | 2020-02-07 | 2020850 | 2020-02-07 | 2020-02-07 | Articles Abstract Database |