DEVELOPMENT AND VALIDATION OF SENSITIVE LC-ESI-MS/M S METHOD FOR THE SIMULTANEOUS ESTIMATION OF DAPAGLIFLOZIN AND SAXAGL IPTIN IN HUMAN PLASMA (Record no. 11176)
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| fixed length control field | a |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20200212095756.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 200212b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 12114 |
| Author | Adluri, Phanindra |
| 245 ## - TITLE STATEMENT | |
| Title | DEVELOPMENT AND VALIDATION OF SENSITIVE LC-ESI-MS/M S METHOD FOR THE SIMULTANEOUS ESTIMATION OF DAPAGLIFLOZIN AND SAXAGL IPTIN IN HUMAN PLASMA |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.11(4) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | M P |
| Name of publisher, distributor, etc. | Innovare Academic Sciences Pvt Ltd |
| Year | 2019 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 55-59p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Objective : To develop and validate a sensitive liquid chromato graphy-electrospray ionization-tandem mass spectrom etric (LC-ESI-MS/MS) technique for the quantification of dapagliflozin a nd saxagliptin in plasma by linagliptin as internal standard. Methods : Chromatography was achieved on hypersil C 18 (50 mmx4 mm) 5 μ analytical column with 0.1% formi c acid and acetonitrile (25:75 V/V) as mobile phase at 0.7 ml/min flow rate. Dapagliflo zin, saxagliptin, and linagliptin were detected at m/z 409.14/135.0, m/z 316.2/180.13 and m/z 472.54/456.21 respectively. Drugs and internal stan dard were extracted by LLE (liquid-liquid extractio n). Results : Developed technique was validated over 0.5-1500.0 n g/ml linear concentration range for dapagliflozin a nd 2.00-2000.0 ng/ml for saxagliptin. This method established with intra-bat ch and inter-batch precision within 2.44-8.12% and 1.25-7.14 % for dapagliflozin and 1.84-7.5 % and 1.02–6.00 % for saxagliptin. This method establ ished with intra-batch and inter-batch accuracy for dapagliflozin within 98.86-103% and 96.98- 102 % respectively and for saxagliptin within 98.05 -109.06 % and 97.00-104.00 % respectively. Conclusion : Both dapagliflozin and saxagliptin were stable duri ng three freeze-thaw cycles, long term and bench-to p stability studies. The developed method was useful for the routine analysi s of dapagliflozin and saxagliptin simultaneously i n plasma samples. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 12115 |
| Co-Author | Shravan Kumar Y. |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | Bhopal Innovare Academic Sciences Pvt Ltd |
| International Standard Serial Number | 2656-0097 |
| Title | International journal of pharmacy and pharmaceutical science |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://innovareacademics.in/journals/index.php/ijpps/article/view/31249/19668 |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Barcode | Date last seen | Price effective from | Koha item type |
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| School of Pharmacy | School of Pharmacy | Archieval Section | 2020-02-12 | 2020909 | 2020-02-12 | 2020-02-12 | Articles Abstract Database |