000 -LEADER |
fixed length control field |
a |
003 - CONTROL NUMBER IDENTIFIER |
control field |
OSt |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20211122121050.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
211122b xxu||||| |||| 00| 0 eng d |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
AIKTC-KRRC |
Transcribing agency |
AIKTC-KRRC |
100 ## - MAIN ENTRY--PERSONAL NAME |
9 (RLIN) |
14719 |
Author |
Mendoza, Ronel Abad |
245 ## - TITLE STATEMENT |
Title |
Bioassay-Guided Isolation and Structure Elucidation of Bioactive Phytoconstituents with Inhibitory Activity against Carbohydrate-Hydrolyzing Enzymes from the Aerial Parts of Premna odorata Blanco |
250 ## - EDITION STATEMENT |
Volume, Issue number |
Vol.55(3), Jul-Sep |
260 ## - PUBLICATION, DISTRIBUTION, ETC. |
Place of publication, distribution, etc. |
Banagalore |
Name of publisher, distributor, etc. |
Association of Pharmaceutical Teachers of India (APTI) |
Year |
2021 |
300 ## - PHYSICAL DESCRIPTION |
Pagination |
846-856p. |
520 ## - SUMMARY, ETC. |
Summary, etc. |
Background: This study aimed to isolate the bioactive components from the aerial parts of Premna odorata Blanco and to evaluate the hypoglycemic potentials of the crude extracts, sub-fractions and final isolate. Materials and Methods: The plant material underwent a series of enzyme assay-guided isolation and purification having the fractions assayed for inhibitory activity against α-amylase using 3,5-dinitrosalicylic acid (DNS) colorimetric method for each stage. H.1 isolate was tested for α-amylase and α-glucosidase inhibitory activity and glucose uptake by yeast cells. Phytochemical characterization and 1H and 13C NMR spectral analysis were done for the structure elucidation of H.1. Results: In vitro amylase studies revealed that at 100 μg/mL concentrations, hexane crude extract, fraction F (20%/80% and 10%/90% n-hexane/dichloromethane fractions) and F.3 (10:1 v/v petroleum ether/ethyl acetate sub-fraction) exhibited 34.38±0.116%, 71.86± 4.909% and 42.16±1.257% inhibition against α-amylase (at 1000 μg/mL concentration), respectively. H.1 isolate exerted significant inhibition (p < 0.05) of 55.99± 2.202% and 72.43±3.988% against α-amylase and α-glucosidase enzyme (both at 1000 μg/ mL concentration), respectively and significant glucose uptake of 13.85± 0.368%. The purified isolate was spectroscopically confirmed as a 5:1 mixture of β- sitosterol (H.1a) and stigmasterol (H.1b). Conclusion: The compounds have significant inhibitory activity against carbohydrate-hydrolyzing enzymes and may be potentially developed as adjuvant pharmacotherapy for type 2 diabetes. |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
9 (RLIN) |
4639 |
Topical term or geographic name entry element |
PHARMACEUTICS |
700 ## - ADDED ENTRY--PERSONAL NAME |
9 (RLIN) |
14720 |
Co-Author |
Shen, Chien-Chang |
773 0# - HOST ITEM ENTRY |
Title |
Indian journal of pharmaceutical education and research |
Place, publisher, and date of publication |
Bengluru Association of Pharmaceutical Teachers of India (APTI) |
International Standard Serial Number |
0019-5464 |
856 ## - ELECTRONIC LOCATION AND ACCESS |
URL |
https://www.ijper.org/sites/default/files/IndJPhaEdRes-55-3-846.pdf |
Link text |
Click here |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
|
Koha item type |
Articles Abstract Database |