Ginsenoside rg5 alleviates 1-methyl-4-phenylpyridinium ion induced parkinson's disease cell model damage by regulating microrna-874-3p/thioredoxin interacting proteins molecular axis (Record no. 17252)
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| 000 -LEADER | |
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| fixed length control field | a |
| 003 - CONTROL NUMBER IDENTIFIER | |
| control field | OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20220730162122.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 220730b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | AIKTC-KRRC |
| Transcribing agency | AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 17455 |
| Author | Zhang, Jidong |
| 245 ## - TITLE STATEMENT | |
| Title | Ginsenoside rg5 alleviates 1-methyl-4-phenylpyridinium ion induced parkinson's disease cell model damage by regulating microrna-874-3p/thioredoxin interacting proteins molecular axis |
| 250 ## - EDITION STATEMENT | |
| Volume, Issue number | Vol.83(6), Nov-Dec |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Place of publication, distribution, etc. | Mumbai |
| Name of publisher, distributor, etc. | Indian Journal of Pharmaceutical Science |
| Year | 2021 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Pagination | 1295-1303p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | This research attempts to probe into the influence mechanism of ginsenoside Rg5 on 1-methyl-4- phenylpyridine ion induced Parkinson's disease cell model damage. Different concentrations (25, 50, 100 μmol/l) of ginsenoside Rg5 acted on human neuroblastoma SK-N-SH cells induced by 1-methyl- 4-phenylpyridine ion. Adopt cell counting kit-8, flow cytometry, quantitative reverse transcription polymerase chain reaction and western blot to exam cell viability, apoptosis rate, microRNA-874-3p and thioredoxin interacting proteins expressions. Transfected microRNA-874-3p mimics and thioredoxin interacting proteins small interfering RNA to SK-N-SH cells, respectively. Then adopt the above mentioned methods to exam microRNA-874-3p overexpression or thioredoxin interacting proteins inhibition influence on SK-N-SH cells viability and apoptosis which is induced by 1-methyl-4-phenylpyridine ion. Adopt dual luciferase report experiment and western blot methods to test the regulatory function of microRNA-874-3p on thioredoxin interacting proteins. After ginsenoside Rg5 acted on 1-methyl-4- phenylpyridine ion induced SK-N-SH cells, the cell viability and microRNA-874-3p expression increased significantly but thioredoxin interacting proteins expression and apoptosis rate decreased significantly (p<0.05). After microRNA-874-3p over-expression, 1-methyl-4-phenylpyridine ion induced SK-N-SH cells viability were significantly increased and apoptosis rate was remarkably reduced (p<0.05). After inhibiting thioredoxin interacting proteins expression, 1-methyl-4-phenylpyridine ion induced SK-N-SH cells viability was significantly increased, but apoptosis rate was remarkably lowered (p<0.05). MicroRNA- 874-3p regulates thioredoxin interacting proteins expression negatively. MicroRNA-874-3p inhibition could attenuate the effect of ginsenoside Rg5 on the viability and apoptosis of 1-methyl-4-phenylpyridine ion induced SK-N-SH (p<0.05). Thioredoxin interacting proteins inhibition went into reverse microRNA- 874-3p inhibition combined with ginsenoside Rg5 treatment on 1-methyl-4-phenylpyridine ion induced SK-N-SH activity and apoptosis (p<0.05). Ginsenoside Rg5 could alleviate 1-methyl-4-phenylpyridine ion induced Parkinson's disease cell model damage and its mechanism possibly has relationship with the up- regulation of microRNA-874-3p/thioredoxin interacting proteins molecular axis. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| 9 (RLIN) | 4639 |
| Topical term or geographic name entry element | PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME | |
| 9 (RLIN) | 17456 |
| Co-Author | Rong, Zhao |
| 773 0# - HOST ITEM ENTRY | |
| Place, publisher, and date of publication | New Delhi |
| Title | Indian journal of pharmaceutical sciences |
| 856 ## - ELECTRONIC LOCATION AND ACCESS | |
| URL | https://www.ijpsonline.com/articles/ginsenoside-rg5-alleviates-1methyl4phenylpyridinium-ion-induced-parkinsons-disease-cell-model-damage-by-regulating-micro.pdf |
| Link text | Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Source of classification or shelving scheme | |
| Koha item type | Articles Abstract Database |
| Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Barcode | Date last seen | Price effective from | Koha item type |
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| School of Pharmacy | School of Pharmacy | Archieval Section | 2022-07-30 | 2022-1254 | 2022-07-30 | 2022-07-30 | Articles Abstract Database |