Impact of microrna-29a-3p on the neural stem cell differentiation process into neurons (Record no. 20855)

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control field 20240406140947.0
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040 ## - CATALOGING SOURCE
Original cataloging agency AIKTC-KRRC
Transcribing agency AIKTC-KRRC
100 ## - MAIN ENTRY--PERSONAL NAME
9 (RLIN) 23188
Author Shi, Xinyu
245 ## - TITLE STATEMENT
Title Impact of microrna-29a-3p on the neural stem cell differentiation process into neurons
250 ## - EDITION STATEMENT
Volume, Issue number Vol.85(4), Jul-Aug
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Mumbai
Name of publisher, distributor, etc. Indian Journal of Pharmaceutical Science
Year 2023
300 ## - PHYSICAL DESCRIPTION
Pagination 1025-1032p.
520 ## - SUMMARY, ETC.
Summary, etc. To explore how microRNA-29a-3p influences the differentiation of neural stem cells into neurons and to
identify any potential mechanisms involved. The program TargetScan, available online was utilized to
examine and make predictions about the microRNA-29a-3p located upstream that regulate doublecortin
X messenger ribonucleic acid. Ultimately, microRNA-29a-3p was preferred as the main central point of the
investigation. The targeting of doublecortin X gene by microRNA-29a-3p was validated by employing a
dual-luciferase reporter gene system. Before subjecting to adherent differentiation, the neural stem cells were
transfected with microRNA-29a-3p inhibitor, microRNA-29a-3p mimic and normal control. MicroRNA and
doublecortin X messenger ribonucleic acid levels were measured through quantitative reverse transcription-
polymerase chain reaction and doublecortin X protein levels were determined through Western blot assays.
The differentiation of neuronal precursor cells was detected by doublecortin X immunofluorescence.
Neuronal differentiation was detected by microtubule-associated protein 2 immunofluorescence. According
to the dual-luciferase reporter gene system, miR-29a-3p targeted the doublecortin X gene; microRNA-29a-
3p significantly decreased in differentiated cells for 3 d was observed, along with a significant reduction in
levels of doublecortin X protein and messenger ribonucleic acid. On the other hand, a significant raise in
microRNA-29a-3p in differentiated cells of microRNA-29a-3p mimic group for a period of 3 d was noticed
with a significant drop in protein levels, doublecortin X messenger ribonucleic acid and doublecortin X
immunofluorescence-positive neuronal precursor cells. From the microtubule-associated protein 2
immunofluorescence, it was confirmed that microRNA-29a-3p inhibitor group significantly elevated the total
number of neurons, while microRNA-29a-3p mimic reduced the neurons on 7 d. Therefore, low expression
of microRNA-29a-3p promote differentiation of neural stem cells into neurons via targeting doublecortin X.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 4639
Topical term or geographic name entry element PHARMACEUTICS
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 23189
Co-Author Zhou, Haonan
773 0# - HOST ITEM ENTRY
Place, publisher, and date of publication New Delhi Indian Pharmaceutical Association
International Standard Serial Number 0250-474X
Title Indian journal of pharmaceutical sciences
856 ## - ELECTRONIC LOCATION AND ACCESS
URL https://www.ijpsonline.com/articles/the-impact-of-microrna29a3p-on-the-neural-stem-cell-differentiation-process-into-neurons.pdf
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          School of Pharmacy School of Pharmacy Archieval Section 2024-04-06 2024-0413 2024-04-06 2024-04-06 Articles Abstract Database
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