| 000 -LEADER |
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a |
| 003 - CONTROL NUMBER IDENTIFIER |
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OSt |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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20190315093418.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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190307b xxu||||| |||| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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AIKTC-KRRC |
| Transcribing agency |
AIKTC-KRRC |
| 100 ## - MAIN ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
7909 |
| Author |
Devi Ramesh |
| 245 ## - TITLE STATEMENT |
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| Title |
Development and validation of RP-HPLC method for the determination of alvimopan in rat plasma |
| 250 ## - EDITION STATEMENT |
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| Volume, Issue number |
Vol. 10(10), July-August |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. |
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| Place of publication, distribution, etc. |
M. P. |
| Name of publisher, distributor, etc. |
Innovare Academic Sciences Pvt Ltd |
| Year |
2018 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Pagination |
124-129 |
| 520 ## - SUMMARY, ETC. |
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| Summary, etc. |
Objective: The present investigation demonstrates a simple, sensitive and accurate high pressure liquid chromatographic (HPLC) method for the determination of alvimopan (AMP) in rat plasma. Methods: The chromatographic separation was achieved within 10 min by using acetonitrile: potassium dihydrogen phosphate buffer pH 3.0 adjusted with orthophosphoric acid (50:50) as mobile phase on Altima Grace Smart C-18 column (5μ; 250 × 4.6 mm) at a flow rate of 1.0 ml/min with injection volume 50 μl. The drug was extracted from plasma by liquid-liquid extraction using a mixture of methanol: acetonitrile (50:50) as a solvent. The retention times of drug and internal standard were found to be 5.17 and 6.74 min, respectively. This method was validated as per the United States Food and Drug Administration (US-FDA) guidelines. Results: The results of the validation parameters were found to be within the acceptance limits. The method was linear in the concentration range from 5-1000 ng/ml (r2= 0.9998), and the extraction recovery was found to be 78.71±3.86% for AMP. The lower limit of quantification was found to be 5ng/ml, and the stability of recovered samples at different conditions was found to be more than 95%. Conclusion: The developed method possess good selectivity, specificity, there was no interference found in the plasma blanks at retention times of AMP and Internal Standard (IS). We found a good correlation between the peak area and con..... |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| 9 (RLIN) |
4639 |
| Topical term or geographic name entry element |
PHARMACEUTICS |
| 700 ## - ADDED ENTRY--PERSONAL NAME |
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| 9 (RLIN) |
7910 |
| Co-Author |
Habibuddin, Mohammad |
| 773 0# - HOST ITEM ENTRY |
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| Title |
International journal of pharmacy and pharmaceutical science |
| International Standard Serial Number |
0975 – 1491 |
| Place, publisher, and date of publication |
Bhopal Innovare Academic Sciences Pvt Ltd |
| 856 ## - ELECTRONIC LOCATION AND ACCESS |
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| URL |
https://innovareacademics.in/journals/index.php/ijpps/article/view/29001/15904 |
| Link text |
Click here |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Source of classification or shelving scheme |
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| Koha item type |
Articles Abstract Database |
