GC-MS Analysis, HPTLC fingerprint profile and DPPH free radical scavenging assay of methanol extract of martynia annua linn seeds (Record no. 9563)
[ view plain ]
000 -LEADER | |
---|---|
fixed length control field | a |
003 - CONTROL NUMBER IDENTIFIER | |
control field | OSt |
005 - DATE AND TIME OF LATEST TRANSACTION | |
control field | 20190930150439.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
fixed length control field | 190930b xxu||||| |||| 00| 0 eng d |
040 ## - CATALOGING SOURCE | |
Original cataloging agency | AIKTC-KRRC |
Transcribing agency | AIKTC-KRRC |
100 ## - MAIN ENTRY--PERSONAL NAME | |
9 (RLIN) | 9665 |
Author | Alrabie, Ali |
245 ## - TITLE STATEMENT | |
Title | GC-MS Analysis, HPTLC fingerprint profile and DPPH free radical scavenging assay of methanol extract of martynia annua linn seeds |
250 ## - EDITION STATEMENT | |
Volume, Issue number | Vol. 11 (06) |
260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
Place of publication, distribution, etc. | Bhopal |
Name of publisher, distributor, etc. | Innovare Academic Sciences Pvt Ltd |
Year | 2019 |
300 ## - PHYSICAL DESCRIPTION | |
Pagination | 14-22p. |
520 ## - SUMMARY, ETC. | |
Summary, etc. | To investigate the photochemical constituents present in methanol extract of martynia annua seeds using Gas Chromatography-Mass Spectroscopy(GC-MS), High-Performance Thin–Layer Chromatography(HPTLC) analysis and study antioxidant activity. Methods: Methanol extract of Martynia annua seeds were subjected to GC-MS and HPTLC analysis. HPTLC analysis was carried out using GAMAG system with a linomate5 applicator, system mobile phase (Toluene: Chloroform: Ethanol (4:4:1 V/V/V)), two different volume of extract was applied 2 µl and 5 µl. GC-MS analysis was carried out on JEOL GC MATE ΙΙ, column HP 5 MS and Quadruple double focusing mass analyzer. Antioxidant activity was determined by DPPH assay. Results: GC-MS analysis provided 17 peaks indicating the presence of seventeen different phytochemicals in methanol extract of martynia annua seeds. HPTLC fingerprint showed 6 peaks at both size 2 µl and 5 µl at 254 nm whereas 4 peaks, 9 peaks were detected at 366 nm for 2 µl and 5 µl respectively. After derivatization with 10 % methanolic sulphuric acid, 8 peaks, 11 peaks were detected for 2 µl and 5 µl respectively when the derivatized plate was scanned at 540 nm. DPPH free radical scavenging result showed EC50 value of 44.1±1.1 µg/ml. Conclusion: The GC-MS analysis showed the presence of fatty acids, ester, aldehydes and ketones whereas in HPTLC different peaks at different UV-lights before and after derivatization were observed. Maximum percentage inhibition using DPPH assay was found 74 at concentration of 50 µg/ml. |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
9 (RLIN) | 4639 |
Topical term or geographic name entry element | PHARMACEUTICS |
700 ## - ADDED ENTRY--PERSONAL NAME | |
9 (RLIN) | 9666 |
Co-Author | Ola Bassar |
773 0# - HOST ITEM ENTRY | |
International Standard Serial Number | 0975 – 1491 |
Place, publisher, and date of publication | Bhopal Innovare Academic Sciences Pvt Ltd |
Title | International journal of pharmacy and pharmaceutical science |
856 ## - ELECTRONIC LOCATION AND ACCESS | |
URL | https://innovareacademics.in/journals/index.php/ijpps/article/view/32206 |
Link text | Click here |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Source of classification or shelving scheme | |
Koha item type | Articles Abstract Database |
Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Barcode | Date last seen | Price effective from | Koha item type |
---|---|---|---|---|---|---|---|---|---|---|---|---|
School of Pharmacy | School of Pharmacy | Archieval Section | 2019-10-11 | 2019846 | 2019-10-11 | 2019-10-11 | Articles Abstract Database |