Anti-tumour activity of bromopyrrole alkaloids against human breast tumour (MCF-7) through apoptosis induction (Record no. 9795)
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fixed length control field | a |
003 - CONTROL NUMBER IDENTIFIER | |
control field | OSt |
005 - DATE AND TIME OF LATEST TRANSACTION | |
control field | 20191019145451.0 |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
fixed length control field | 191019b xxu||||| |||| 00| 0 eng d |
040 ## - CATALOGING SOURCE | |
Original cataloging agency | AIKTC-KRRC |
Transcribing agency | AIKTC-KRRC |
100 ## - MAIN ENTRY--PERSONAL NAME | |
9 (RLIN) | 10010 |
Author | Manikandan, S. |
245 ## - TITLE STATEMENT | |
Title | Anti-tumour activity of bromopyrrole alkaloids against human breast tumour (MCF-7) through apoptosis induction |
250 ## - EDITION STATEMENT | |
Volume, Issue number | Vol. 11 (06) |
260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
Place of publication, distribution, etc. | Bhopal |
Name of publisher, distributor, etc. | Innovare Academic Sciences Pvt Ltd |
Year | 2019 |
300 ## - PHYSICAL DESCRIPTION | |
Pagination | 94-100 |
520 ## - SUMMARY, ETC. | |
Summary, etc. | The present study was conducted to screen the cytotoxicity and initiation of apoptosis in MCF-7 human breast tumour cells using the Indian sponge Acanthostylotela cornuta of the Gulf of Mannar. Methods: The crude methanol extract of A. cornuta was fractionated using a reversed phase silica gel column chromatography. The purity of the active fraction of bromopyrrole alkaloids was confirmed with Gas Chromatography-Mass Spectrometry (GCMS). The bromopyrrole alkaloids induce apoptotic changes in MCF-7 cells were studied with electrophoresis, caspase assay, and different staining analysis. The MCF-7 cells were analyzed by Flow cytometry to determine their DNA content. Results: Bromopyrrole alkaloids of A. cornuta showed cytotoxicity against MCF-7 human breast tumour cells with the IC50 value of 8.0μg/ml. The bromopyrrole compounds induced cells exhibited scatted red fluorescence, showing the presence of several residual bodies and condensation of chromatin. Caspase-3, pro-caspase-9, caspase-9 and Poly-ADP-Ribose-Polymerase (PARP) activity were occurred in bromopyrrole alkaloids treated tumour cell. The cell cycle arrest is conceivable that the compound prevented the progression of cell cycle through the G phase resulting from inhibition of survival, leading them to undergo apoptosis. Conclusion: Bromopyrrole alkaloids of A. cornuta possess antitumor activity which was arrested the G phase in cell cycle that clearly indicated its nature as that of antitumour drugs. |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
9 (RLIN) | 4639 |
Topical term or geographic name entry element | PHARMACEUTICS |
700 ## - ADDED ENTRY--PERSONAL NAME | |
9 (RLIN) | 10011 |
Co-Author | Ganesapandian, S. |
773 0# - HOST ITEM ENTRY | |
International Standard Serial Number | 2656-0097 |
Title | International journal of pharmacy and pharmaceutical science |
Place, publisher, and date of publication | Bhopal Innovare Academic Sciences Pvt Ltd |
856 ## - ELECTRONIC LOCATION AND ACCESS | |
URL | https://innovareacademics.in/journals/index.php/ijpps/article/view/29092 |
Link text | Click here |
942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
Source of classification or shelving scheme | |
Koha item type | Articles Abstract Database |
Withdrawn status | Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Permanent Location | Current Location | Shelving location | Date acquired | Barcode | Date last seen | Price effective from | Koha item type |
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School of Pharmacy | School of Pharmacy | Archieval Section | 2019-10-19 | 2019948 | 2019-10-19 | 2019-10-19 | Articles Abstract Database |