Determination of vindoline and rutin content in five different morphotypes of catharanthus roseus leaves using HPLC (Record no. 9921)

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005 - DATE AND TIME OF LATEST TRANSACTION
control field 20191101113159.0
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fixed length control field 191101b xxu||||| |||| 00| 0 eng d
040 ## - CATALOGING SOURCE
Original cataloging agency AIKTC-KRRC
Transcribing agency AIKTC-KRRC
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9 (RLIN) 10219
Author Kapoor, Manish
245 ## - TITLE STATEMENT
Title Determination of vindoline and rutin content in five different morphotypes of catharanthus roseus leaves using HPLC
250 ## - EDITION STATEMENT
Volume, Issue number Vol. 11(8)
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Place of publication, distribution, etc. Bhopal
Name of publisher, distributor, etc. Innovare Academic Sciences Pvt Ltd
Year 2019
300 ## - PHYSICAL DESCRIPTION
Pagination 52-57p.
520 ## - SUMMARY, ETC.
Summary, etc. Objective: To determine total phenolic and flavonoids contents and also quantify vindoline and rutin in different morphotypes of Catharanthus roseus using High-performance liquid chromatography (HPLC) method.

Methods: Total flavonoids content (TFC) was determined by Aluminium chloride colorimetric and total phenolic content (TPC) was estimated by Folin-Ciocalteu reagent assay. The chromatographic separation was done by using a C18 column at room temperature and eluted with a mobile phase consisting of a mixture of phosphate buffer (pH=5.8) and acetonitrile at a flow rate of 1.0 ml/minute and detection was carried out at 254 nm.

Results: TPC and TFC content was found highest in Cr00DP and lowest in Cr00WFSRE. Results also showed that the purple morphotypes Cr00DP gives more vindoline (0.3 mg/g) and rutin (18.57 mg/g) concentration compared to the pink morphotype Cr00PFRE contained 18.3 mg/g rutin and 0.2 mg/g vindoline. White morphotypes contained 0.383 mg/g rutin and 0.004 mg/g vindoline which was significantly less as compared to purple and pink morphotypes.

Conclusion: The plant has significant number of alkaloids and flavonoids. The obtained outcomes from different morphotypes are thus significant for the purpose of vindoline and rutin isolation from Catharanthus roseus plant. These isolated bioactive phytoconstituents are a good candidate for further pharmacological and clinical study.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
9 (RLIN) 4639
Topical term or geographic name entry element PHARMACEUTICS
700 ## - ADDED ENTRY--PERSONAL NAME
9 (RLIN) 10220
Co-Author Rani, Jyoti
773 0# - HOST ITEM ENTRY
Place, publisher, and date of publication Bhopal Innovare Academic Sciences Pvt Ltd
International Standard Serial Number 2656-0097
Title International journal of pharmacy and pharmaceutical science
856 ## - ELECTRONIC LOCATION AND ACCESS
URL https://innovareacademics.in/journals/index.php/ijpps/article/view/31140
Link text Click here
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Koha item type Articles Abstract Database
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          School of Pharmacy School of Pharmacy Archieval Section 2019-11-01 2020039 2019-11-01 2019-11-01 Articles Abstract Database
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