ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR SI MULTANEOUS ESTIMATION OF CURCUMIN AND CYCLOSPORINE BY RP-HPLC
By: Desai, Neha
.
Contributor(s): Momin, Munira.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol.11(2).Description: 26-33p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective:
The present work was undertaken with an aim to deve
lop and validate a rapid reverse-phase high-perform
ance liquid chromatography
(RP-HPLC) method for the estimation of curcumin and
cyclosporine in the capsule dosage form.
Methods:
The RP-HPLC method for the simultaneous estimation o
f curcumin and cyclosporine was developed using Agi
lent (Infinity 1260) HPLC
system and Eclipse XDB-C18 (4.6 x 150 mm i.d., 5μ)
stationary phase. The optimized mobile phase compri
sed of acetonitrile: water: methanol (50:
10: 40 v/v/v) pumped at a flow rate of 0.5 ml/min.
Separation of drugs was achieved in an isocratic mo
de and elution was monitored using PDA
detector at 214 nm. The method was validated as per
ICH-Q2R1 guidelines.
Results:
Retention time of the curcumin and cyclosporine wer
e found to be 3.073 min and 6.373 min with the corr
elation coefficient (R
2
) of 0.9993
and 0.998 respectively. The response of curcumin an
d cyclosporine was found linear in the concentratio
n range of 8-48 μg/ml and 4-24 μg/ml
respectively. The percent recovery values were foun
d in the range of 97-103% indicating satisfactory a
ccuracy of the method. The percent relative
standard deviation (% RSD) values for the precision
study was less than 2 which suggest that the metho
d is precise.
Conclusion:
The proposed method was found accurate, precise and
specific for the determination of curcumin and cyc
losporine in bulk as well as in
capsule dosage form. Thus, the present method can b
e used for routine analysis and quality control of
curcumin and cyclosporine in bulk and
capsule dosage form.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2020884 |
Objective:
The present work was undertaken with an aim to deve
lop and validate a rapid reverse-phase high-perform
ance liquid chromatography
(RP-HPLC) method for the estimation of curcumin and
cyclosporine in the capsule dosage form.
Methods:
The RP-HPLC method for the simultaneous estimation o
f curcumin and cyclosporine was developed using Agi
lent (Infinity 1260) HPLC
system and Eclipse XDB-C18 (4.6 x 150 mm i.d., 5μ)
stationary phase. The optimized mobile phase compri
sed of acetonitrile: water: methanol (50:
10: 40 v/v/v) pumped at a flow rate of 0.5 ml/min.
Separation of drugs was achieved in an isocratic mo
de and elution was monitored using PDA
detector at 214 nm. The method was validated as per
ICH-Q2R1 guidelines.
Results:
Retention time of the curcumin and cyclosporine wer
e found to be 3.073 min and 6.373 min with the corr
elation coefficient (R
2
) of 0.9993
and 0.998 respectively. The response of curcumin an
d cyclosporine was found linear in the concentratio
n range of 8-48 μg/ml and 4-24 μg/ml
respectively. The percent recovery values were foun
d in the range of 97-103% indicating satisfactory a
ccuracy of the method. The percent relative
standard deviation (% RSD) values for the precision
study was less than 2 which suggest that the metho
d is precise.
Conclusion:
The proposed method was found accurate, precise and
specific for the determination of curcumin and cyc
losporine in bulk as well as in
capsule dosage form. Thus, the present method can b
e used for routine analysis and quality control of
curcumin and cyclosporine in bulk and
capsule dosage form.
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