VALIDATED RP -HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF PYRANTEL PAMOATE AND PRAZIQUANTEL IN BULK AND PHARMACEUTICAL DOSAGE FORM
By: Rajesh, R
.
Contributor(s): James,Jithu Jerin.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2019Edition: Vol.1(5).Description: 62-67p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective:
To develop a simple, accurate and precise
reverse-
phase high
-performance liquid chromatography (
RP
-HPLC
) metho
d and subsequently
validate
for the simultaneous estimation of praziquantel (PZ
Q) and pyrantel pamoate (PP) in the
pharmaceutical dosage form.
Method
s:
The chromatographic separation was achieved on Phenomenex Luna C
18
Result
s:
The retention time of PZQ and PP was found to be 3.897 min and 1.697 min re
spectively. The method was validated in terms of specificity,
accuracy, precision, linearity and robustness as per ICH guidelines. Linearity was obtained in the concentration range of
20
–
60 μg
/ml
for both PZQ
and PP with correlation coefficients of 0.987 and 0.998 respectively. The accuracy of the method was determined using a recov
ery test and found as
98.44 % to 100.35 %. All parameters are found to be within the acceptable limit.
column (250 mm × 4.6 mm, 5
μm) as st
ationary phase
maintained at an ambient temperature with a mobile phase comprising of wate
r:
acetonitrile (20
: 80) at a flow rate of 1.0 ml/min and UV detection
at 220 nm.
Conclu
sio
n:
The developed RP
-HPLC method was simple, rapid, accurate, precise for the simultaneous estimation of PZQ and PP in bulk and tablet
dosage form
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2020929 |
Objective:
To develop a simple, accurate and precise
reverse-
phase high
-performance liquid chromatography (
RP
-HPLC
) metho
d and subsequently
validate
for the simultaneous estimation of praziquantel (PZ
Q) and pyrantel pamoate (PP) in the
pharmaceutical dosage form.
Method
s:
The chromatographic separation was achieved on Phenomenex Luna C
18
Result
s:
The retention time of PZQ and PP was found to be 3.897 min and 1.697 min re
spectively. The method was validated in terms of specificity,
accuracy, precision, linearity and robustness as per ICH guidelines. Linearity was obtained in the concentration range of
20
–
60 μg
/ml
for both PZQ
and PP with correlation coefficients of 0.987 and 0.998 respectively. The accuracy of the method was determined using a recov
ery test and found as
98.44 % to 100.35 %. All parameters are found to be within the acceptable limit.
column (250 mm × 4.6 mm, 5
μm) as st
ationary phase
maintained at an ambient temperature with a mobile phase comprising of wate
r:
acetonitrile (20
: 80) at a flow rate of 1.0 ml/min and UV detection
at 220 nm.
Conclu
sio
n:
The developed RP
-HPLC method was simple, rapid, accurate, precise for the simultaneous estimation of PZQ and PP in bulk and tablet
dosage form
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