New Validated Diffuse Reflectance Infrared Fourier Transform Spectroscopic Method for the Quantification of Levofloxacin in Pharmaceutical Dosage Form
By: ALNAQBI, A. M. S
.
Contributor(s): BHONGADE, B. A.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(3), May-June.Description: 430-436p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: Levofloxacin is a synthetic fluoroquinolone antibiotic available in different dosage forms for the treatment
of various bacterial infections. Here in, we report the validated diffuse reflectance infrared Fourier
transform spectroscopic method for quantitative analysis of levofloxacin in the solid dosage forms. This
method offers the advantage of being eco-friendly that is applied for solid state samples. Appropriate
quantities of levofloxacin and dry potassium bromide were mixed to get the desired concentration of samples
for Fourier transform infrared spectroscopic analysis using a diffuse reflectance sampling interface. The
peak in the Fourier transform infrared spectrum around 1724 cm-1 was observed for the carbonyl group
of levofloxacin. The calibration curve was plotted for the area under the curve of carbonyl peak against
the concentration of calibration standards. A linear calibration curve with a correlation coefficient of 0.999
was obtained in the range of 0.3-1.8 % w/w of levofloxacin. The calibration curve method was linear,
precise and accurate as per guidelines. The method employed for quantification of levofloxacin in three
marketed tablet dosage forms and laboratory physical mixture yielded a mean recovery of 101.32 and
98.11 percent by weight, respectively. Overall, the precision, accuracy and reproducibility of the proposed
diffuse reflectance infrared Fourier transform spectroscopy method make it appropriate for quantitative
analysis of levofloxacin in solid dosage forms.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
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Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2021-2022550 |
Levofloxacin is a synthetic fluoroquinolone antibiotic available in different dosage forms for the treatment
of various bacterial infections. Here in, we report the validated diffuse reflectance infrared Fourier
transform spectroscopic method for quantitative analysis of levofloxacin in the solid dosage forms. This
method offers the advantage of being eco-friendly that is applied for solid state samples. Appropriate
quantities of levofloxacin and dry potassium bromide were mixed to get the desired concentration of samples
for Fourier transform infrared spectroscopic analysis using a diffuse reflectance sampling interface. The
peak in the Fourier transform infrared spectrum around 1724 cm-1 was observed for the carbonyl group
of levofloxacin. The calibration curve was plotted for the area under the curve of carbonyl peak against
the concentration of calibration standards. A linear calibration curve with a correlation coefficient of 0.999
was obtained in the range of 0.3-1.8 % w/w of levofloxacin. The calibration curve method was linear,
precise and accurate as per guidelines. The method employed for quantification of levofloxacin in three
marketed tablet dosage forms and laboratory physical mixture yielded a mean recovery of 101.32 and
98.11 percent by weight, respectively. Overall, the precision, accuracy and reproducibility of the proposed
diffuse reflectance infrared Fourier transform spectroscopy method make it appropriate for quantitative
analysis of levofloxacin in solid dosage forms.
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