Experimental Design Methodology for Simultaneous Determination of Anti-Diabetic Drugs by Reverse Phase Liquid Chromatographic Method
By: BUKKAPATNAM, V
.
Contributor(s): MUKTHINUTHALAPATI, MATHRUSRI ANNAPURNA.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(3), May-June.Description: 523-534p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: A liquid chromatographic method has been established for the separation of metformin, glipizide,
gliclazide, glibenclamide and glimepiride using experimental design. The main intention of this method is
to optimize suitable chromatographic conditions for the proper elution of the drug molecules with minimal
analysis time. The relationship between the individual and combined effect of critical process parameters
and chromatographic efficiency was made clear and was achieved with the aid of experimental design
methodology. Waters Sunfire C18 type column (150×4.6 mm, 5 μm particle size) was employed and 0.1
% acetic acid in water: acetonitrile mixture was adopted as mobile phase (flow rate: 0.469 ml/min) for
the separation of analytes. The developed method was validated and has been extended for the assay of
marketed formulations.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
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Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2021-2022560 |
A liquid chromatographic method has been established for the separation of metformin, glipizide,
gliclazide, glibenclamide and glimepiride using experimental design. The main intention of this method is
to optimize suitable chromatographic conditions for the proper elution of the drug molecules with minimal
analysis time. The relationship between the individual and combined effect of critical process parameters
and chromatographic efficiency was made clear and was achieved with the aid of experimental design
methodology. Waters Sunfire C18 type column (150×4.6 mm, 5 μm particle size) was employed and 0.1
% acetic acid in water: acetonitrile mixture was adopted as mobile phase (flow rate: 0.469 ml/min) for
the separation of analytes. The developed method was validated and has been extended for the assay of
marketed formulations.
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