Effects of Recombinant Human Erythropoietin on Inflammatory Factors in Rats with Traumatic Brain Injury
By: XUE, KAI
.
Contributor(s): JIN, ZHENG.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(3), May-June.Description: 541-546p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: To study the effects of recombinant human erythropoietin on inflammatory factors in rats with traumatic
brain injury is the main objective. A total of 45 specific-pathogen-free grade male Sprague-Dawley rats
were randomly assigned into sham operation group (sham group), model group and recombinant human
erythropoietin intervention group (treatment group) (n=15). Model and treatment groups were prepared
into traumatic brain injury model by hitting the head through the modified Feeney’s free-fall impact
method, while the head of sham group was not hit. After modeling, treatment group was intraperitoneally
injected with recombinant human erythropoietin at 5000 IU/kg daily and sham and model groups were
intraperitoneally injected with the same dose of normal saline. The rats were killed after 7 d of continuous
administration. The changes of brain mitochondrial membrane potential were detected through rhodamine
123 staining and immunocytochemistry and Western blotting were separately employed to measure the
expressions of interleukin-1β, interleukin-6 and tumor necrosis factor-α in brain tissues and the expression
levels of dynamin-related protein 1, fission 1, mitofusin 2 and optic atrophy 1, mitochondrial dynamics
related proteins in brain tissues. Compared with sham group, model group exhibited significantly weakened
rhodamine 123 fluorescence intensity, increased expressions of interleukin-1β, interleukin-6 and tumor
necrosis factor-α, dynamin-related protein 1 and fission 1 and reduced expressions of mitofusin 2 and optic
atrophy 1 in brain tissues (p<0.05). In comparison with model group, treatment group had significantly
enhanced rhodamine 123 fluorescence intensity, reduced expressions of interleukin-1β, interleukin-6 and
tumor necrosis factor-α, dynamin-related protein 1 and fission 1 and elevated expressions of mitofusin 2
and optic atrophy 1 in brain tissues (p<0.05). Recombinant human erythropoietin can protect the brain
after traumatic brain injury by relieving the inflammatory response and mitochondrial injury after
traumatic brain injury.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2021-2022563 |
To study the effects of recombinant human erythropoietin on inflammatory factors in rats with traumatic
brain injury is the main objective. A total of 45 specific-pathogen-free grade male Sprague-Dawley rats
were randomly assigned into sham operation group (sham group), model group and recombinant human
erythropoietin intervention group (treatment group) (n=15). Model and treatment groups were prepared
into traumatic brain injury model by hitting the head through the modified Feeney’s free-fall impact
method, while the head of sham group was not hit. After modeling, treatment group was intraperitoneally
injected with recombinant human erythropoietin at 5000 IU/kg daily and sham and model groups were
intraperitoneally injected with the same dose of normal saline. The rats were killed after 7 d of continuous
administration. The changes of brain mitochondrial membrane potential were detected through rhodamine
123 staining and immunocytochemistry and Western blotting were separately employed to measure the
expressions of interleukin-1β, interleukin-6 and tumor necrosis factor-α in brain tissues and the expression
levels of dynamin-related protein 1, fission 1, mitofusin 2 and optic atrophy 1, mitochondrial dynamics
related proteins in brain tissues. Compared with sham group, model group exhibited significantly weakened
rhodamine 123 fluorescence intensity, increased expressions of interleukin-1β, interleukin-6 and tumor
necrosis factor-α, dynamin-related protein 1 and fission 1 and reduced expressions of mitofusin 2 and optic
atrophy 1 in brain tissues (p<0.05). In comparison with model group, treatment group had significantly
enhanced rhodamine 123 fluorescence intensity, reduced expressions of interleukin-1β, interleukin-6 and
tumor necrosis factor-α, dynamin-related protein 1 and fission 1 and elevated expressions of mitofusin 2
and optic atrophy 1 in brain tissues (p<0.05). Recombinant human erythropoietin can protect the brain
after traumatic brain injury by relieving the inflammatory response and mitochondrial injury after
traumatic brain injury.
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