Effects of Ambroxol Hydrochloride on Lung Tissue Cell Apoptosis and Vascular Remodeling in Rats with Smoke Induced Chronic Obstructive Pulmonary Disease
By: XIE, JING
.
Contributor(s): QIN, YEYU.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(3), May-June.Description: 594-602p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: To evaluate the effects of ambroxol hydrochloride on lung tissue cell apoptosis and vascular remodeling
in rats with smoke induced chronic obstructive pulmonary disease. Sprague Dawley rats were randomly
divided into normal, model, experimental and control groups (n=20). The rat model of chronic obstructive
pulmonary disease was established by fumigation using Yan’an Cigarettes for 64 d. After fumigation, the
experimental and control groups were subcutaneously injected with 5 ml of Ambroxol hydrochloride (20
mg/kg) and 5 ml of Bambuterol hydrochloride (20 mg/kg) respectively, while the normal and model groups
were intraperitoneally injected with an equal dose of normal saline. Following drug intervention for 28
d, the pathological changes in lung tissues, vascular remodeling, lung tissue cell apoptosis, expressions
of alpha-smooth muscle actin and vascular endothelial growth factor, as well as levels of caspase-3,
B-cell lymphoma-2 and B-cell lymphoma-2 associated X protein were detected by hematoxylin and eosin
staining, elastic Van Gieson staining, terminal deoxynucleotidyl transferase dUTP nick end labeling
staining, immunohistochemical staining and western blotting, respectively. Compared with normal
group, the damage of lung tissues was obvious, the medial thickness of pulmonary arterioles significantly
increased, the degree of vascular muscularization, apoptosis rate and expressions of alpha-smooth muscle
actin, vascular endothelial growth factor, caspase-3 and B-cell lymphoma-2 associated X protein rose and
the expression of B-cell lymphoma-2 decreased in model group (p<0.05). Compared with model group,
the damage of lung tissues was significantly improved, the medial thickness of pulmonary arterioles
decreased, the degree of vascular muscularization, apoptosis rate and expressions of alpha-smooth muscle
actin, vascular endothelial growth factor, caspase-3 and B-cell lymphoma-2 associated X protein reduced
and the expression of B-cell lymphoma-2 rose in experimental and control groups (p<0.05). Ambroxol
hydrochloride can inhibit the apoptosis of lung tissue cells and improve vascular remodeling, thereby
protecting the lung tissues of chronic obstructive pulmonary disease rats.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2021-2022572 |
To evaluate the effects of ambroxol hydrochloride on lung tissue cell apoptosis and vascular remodeling
in rats with smoke induced chronic obstructive pulmonary disease. Sprague Dawley rats were randomly
divided into normal, model, experimental and control groups (n=20). The rat model of chronic obstructive
pulmonary disease was established by fumigation using Yan’an Cigarettes for 64 d. After fumigation, the
experimental and control groups were subcutaneously injected with 5 ml of Ambroxol hydrochloride (20
mg/kg) and 5 ml of Bambuterol hydrochloride (20 mg/kg) respectively, while the normal and model groups
were intraperitoneally injected with an equal dose of normal saline. Following drug intervention for 28
d, the pathological changes in lung tissues, vascular remodeling, lung tissue cell apoptosis, expressions
of alpha-smooth muscle actin and vascular endothelial growth factor, as well as levels of caspase-3,
B-cell lymphoma-2 and B-cell lymphoma-2 associated X protein were detected by hematoxylin and eosin
staining, elastic Van Gieson staining, terminal deoxynucleotidyl transferase dUTP nick end labeling
staining, immunohistochemical staining and western blotting, respectively. Compared with normal
group, the damage of lung tissues was obvious, the medial thickness of pulmonary arterioles significantly
increased, the degree of vascular muscularization, apoptosis rate and expressions of alpha-smooth muscle
actin, vascular endothelial growth factor, caspase-3 and B-cell lymphoma-2 associated X protein rose and
the expression of B-cell lymphoma-2 decreased in model group (p<0.05). Compared with model group,
the damage of lung tissues was significantly improved, the medial thickness of pulmonary arterioles
decreased, the degree of vascular muscularization, apoptosis rate and expressions of alpha-smooth muscle
actin, vascular endothelial growth factor, caspase-3 and B-cell lymphoma-2 associated X protein reduced
and the expression of B-cell lymphoma-2 rose in experimental and control groups (p<0.05). Ambroxol
hydrochloride can inhibit the apoptosis of lung tissue cells and improve vascular remodeling, thereby
protecting the lung tissues of chronic obstructive pulmonary disease rats.
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