Evaluation of anticancer activity of Clerodendrum viscosum leaves against breast carcinoma
By: Shendge, Anil Khushalrao
.
Contributor(s): Basu, Tapasree.
Publisher: Mumbai Wolter Kluwer 2021Edition: Vol.53(5), Sep-Oct.Description: 377-383p.Subject(s): PHARMACOLOGYOnline resources: Click here
In:
Indian Journal of PharmacologySummary: INTRODUCTION: The use of natural resources as medicines for cancer therapies has been described
throughout history in the form of traditional medicines. However, many resources are still unidentified
for their potent biological activities.Clerodendrum viscosum is a hill glory bower reported as a remedy
against oxidative stress, skin diseases, and intestinal infections.
MATERIALS AND METHODS: We have collected the C. viscosum leaves and used for the
preparation of 70% methanolic extract (CVLME). Then, CVLME has been confirmed for anticancer
properties on various cancer cell lines by evaluating cytotoxicity, cell cycle analysis, induction of
ROS and apoptosis, and nuclear fragmentation. Further, the phytochemical analysis of CVLME was
evaluated through high‑performance liquid chromatography.
RESULTS: Cell proliferation assay revealed the selective cytotoxicity of CVLME against breast
cancer cell line (MCF‑7). The FACS‑based cell cycle analysis showed increased subG1 (apoptosis)
population dose dependently. Further, the apoptosis‑inducing effect of CVLME was confirmed by
annexin staining. Flow cytometry and confocal microscopy revealed the selective ROS generation
upon CVLME treatment. The confocal‑based morphological study also revealed condensed and
fragmented nuclear structure in CVLME‑treated MCF‑7 cells. Phytochemical investigations further
indicated the presence of tannic acid, catechin, rutin, and reserpine which might be the reason for
the anticancer activity of CVLME.
CONCLUSION: The above‑combined results revealed the anticancer effect of CVLME, which may
be due to the selective induction of ROS in breast carcinoma
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2021-2022677 |
INTRODUCTION: The use of natural resources as medicines for cancer therapies has been described
throughout history in the form of traditional medicines. However, many resources are still unidentified
for their potent biological activities.Clerodendrum viscosum is a hill glory bower reported as a remedy
against oxidative stress, skin diseases, and intestinal infections.
MATERIALS AND METHODS: We have collected the C. viscosum leaves and used for the
preparation of 70% methanolic extract (CVLME). Then, CVLME has been confirmed for anticancer
properties on various cancer cell lines by evaluating cytotoxicity, cell cycle analysis, induction of
ROS and apoptosis, and nuclear fragmentation. Further, the phytochemical analysis of CVLME was
evaluated through high‑performance liquid chromatography.
RESULTS: Cell proliferation assay revealed the selective cytotoxicity of CVLME against breast
cancer cell line (MCF‑7). The FACS‑based cell cycle analysis showed increased subG1 (apoptosis)
population dose dependently. Further, the apoptosis‑inducing effect of CVLME was confirmed by
annexin staining. Flow cytometry and confocal microscopy revealed the selective ROS generation
upon CVLME treatment. The confocal‑based morphological study also revealed condensed and
fragmented nuclear structure in CVLME‑treated MCF‑7 cells. Phytochemical investigations further
indicated the presence of tannic acid, catechin, rutin, and reserpine which might be the reason for
the anticancer activity of CVLME.
CONCLUSION: The above‑combined results revealed the anticancer effect of CVLME, which may
be due to the selective induction of ROS in breast carcinoma
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