Cadmium chloride and silver nitrate affect the gene expression, stevioside and rebaudioside a production in stevia rebaudiana (bert.)
By: Tahmasi, S
.
Contributor(s): Garoosi, G. A.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(4), July-Aug.Description: 663-670p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: Stevia rebaudiana (Bert.) produces steviol glycoside sweeteners that are sweeter than sucrose. Stevioside
and rebaudioside A are important steviol glycosides used in food production. This study was conducted
to investigate the effect of different concentrations of cadmium chloride (0, 20, 40, 60 and 80 mg/l) and
silver nitrate (0, 15, 30, 45 and 60 mg/l) and different sampling times on stevioside and rebaudioside A
production and Ent-kaurenoic acid 13-hydroxylase, uridine diphosphate-glycosyltransferase 74G1 and
uridine diphosphate-glycosyltransferase 76G1 genes expression in in vitro conditions. The results showed
that the application of cadmium nitrate and silver nitrate decreased stevioside and rebaudioside A
production. Among various concentrations of cadmium chloride, the highest concentrations of stevioside
and rebaudioside A were respectively 39.76 mg/g and 2.27 mg/l dry weight 96 and 72 h after elicitation with
20 mg/l cadmium chloride. Among different concentrations of silver nitrate, the highest amounts of stevioside
and rebaudioside A were respectively 33.33 mg/g dry weight was 2.19 mg/l obtained at control condition
after 96 h and elicitation with 60 mg/l for 24 h. In elicitation with cadmium chloride the expression all
three genes were increased but silver nitrate elicitation increased uridine diphosphate-glycosyltransferase
76G1 gene expression and decreased the Ent-kaurenoic acid 13-hydroxylase and uridine diphosphate-
glycosyltransferase 74G1 genes expression. The correlation analysis showed that Ent-kaurenoic acid
13-hydroxylase gene expression was not correlated with uridine diphosphate-glycosyltransferase
74G1 and uridine diphosphate-glycosyltransferase 76G1 genes expression, while uridine diphosphate-
glycosyltransferase 74G1 was inversely correlated with uridine diphosphate-glycosyltransferase 76G1.
Also, positive correlations were observed between uridine diphosphate-glycosyltransferase 74G1 gene
expression with stevioside synthesis and uridine diphosphate-glycosyltransferase 76G1 gene expression
with rebaudioside A.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-0915 |
Stevia rebaudiana (Bert.) produces steviol glycoside sweeteners that are sweeter than sucrose. Stevioside
and rebaudioside A are important steviol glycosides used in food production. This study was conducted
to investigate the effect of different concentrations of cadmium chloride (0, 20, 40, 60 and 80 mg/l) and
silver nitrate (0, 15, 30, 45 and 60 mg/l) and different sampling times on stevioside and rebaudioside A
production and Ent-kaurenoic acid 13-hydroxylase, uridine diphosphate-glycosyltransferase 74G1 and
uridine diphosphate-glycosyltransferase 76G1 genes expression in in vitro conditions. The results showed
that the application of cadmium nitrate and silver nitrate decreased stevioside and rebaudioside A
production. Among various concentrations of cadmium chloride, the highest concentrations of stevioside
and rebaudioside A were respectively 39.76 mg/g and 2.27 mg/l dry weight 96 and 72 h after elicitation with
20 mg/l cadmium chloride. Among different concentrations of silver nitrate, the highest amounts of stevioside
and rebaudioside A were respectively 33.33 mg/g dry weight was 2.19 mg/l obtained at control condition
after 96 h and elicitation with 60 mg/l for 24 h. In elicitation with cadmium chloride the expression all
three genes were increased but silver nitrate elicitation increased uridine diphosphate-glycosyltransferase
76G1 gene expression and decreased the Ent-kaurenoic acid 13-hydroxylase and uridine diphosphate-
glycosyltransferase 74G1 genes expression. The correlation analysis showed that Ent-kaurenoic acid
13-hydroxylase gene expression was not correlated with uridine diphosphate-glycosyltransferase
74G1 and uridine diphosphate-glycosyltransferase 76G1 genes expression, while uridine diphosphate-
glycosyltransferase 74G1 was inversely correlated with uridine diphosphate-glycosyltransferase 76G1.
Also, positive correlations were observed between uridine diphosphate-glycosyltransferase 74G1 gene
expression with stevioside synthesis and uridine diphosphate-glycosyltransferase 76G1 gene expression
with rebaudioside A.
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