Curcumin induces human glioma cell apoptosis by promoting reactive oxygen species production
By: Zhou, Yu
.
Contributor(s): Liu, Li.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(4), Jul-Aug.Description: 714-722p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: Malignant glioma by surgery is difficult to completely remove, the recurrence rate is high, the survival
period is short and the prognosis is poor, which poses a great threat to human health. This article mainly
studies the related process that curcumin induces human glioma cell apoptosis by promoting reactive
oxygen species production. In the experiment, glioma cells were processed and Uppsala 87 malignant
glioma cells of human were cultured in vitro. 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium
bromide assay was used to detect the effect of curcumin on the proliferation of Uppsala 87 malignant
glioma cells. The cells in each reagent group and control group treated with curcumin were treated with
3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide colorimetric treatment at 24 h. Use flow
cytometry to detect cell apoptosis. Cellular immunofluorescence was used to detect the effect of oxidase
inhibitors on nuclear factor activation. Experimental data showed that the apoptotic rate of 0, 2.5,
5 mg/l, diallyl disulphide treatment groups were 12.3 %, 19.75 % and 26.44 %, respectively, indicating
that diallyl disulphide caused an increase in the apoptotic rate of human promyelocytic leukemia cell line
cells and the difference was statistically significant (p<0.05). The results show that curcumin can inhibit
the proliferation of Uppsala 87 malignant glioma cells cultured in vitro, induce the differential expression
of apoptosis-related genes and thus have a significant effect of promoting cell apoptosiss.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-0920 |
Malignant glioma by surgery is difficult to completely remove, the recurrence rate is high, the survival
period is short and the prognosis is poor, which poses a great threat to human health. This article mainly
studies the related process that curcumin induces human glioma cell apoptosis by promoting reactive
oxygen species production. In the experiment, glioma cells were processed and Uppsala 87 malignant
glioma cells of human were cultured in vitro. 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium
bromide assay was used to detect the effect of curcumin on the proliferation of Uppsala 87 malignant
glioma cells. The cells in each reagent group and control group treated with curcumin were treated with
3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide colorimetric treatment at 24 h. Use flow
cytometry to detect cell apoptosis. Cellular immunofluorescence was used to detect the effect of oxidase
inhibitors on nuclear factor activation. Experimental data showed that the apoptotic rate of 0, 2.5,
5 mg/l, diallyl disulphide treatment groups were 12.3 %, 19.75 % and 26.44 %, respectively, indicating
that diallyl disulphide caused an increase in the apoptotic rate of human promyelocytic leukemia cell line
cells and the difference was statistically significant (p<0.05). The results show that curcumin can inhibit
the proliferation of Uppsala 87 malignant glioma cells cultured in vitro, induce the differential expression
of apoptosis-related genes and thus have a significant effect of promoting cell apoptosiss.
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