Development and validation by reverse phase high performance liquid chromatography method for the estimation of piperine and coenzyme q10 in bulk and pharmaceutical dosage form
By: Sarkar, M
.
Contributor(s): Bag, Puja.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(6), Nov-Dec.Description: 1208-1214p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: Today people are focused on their health, as good health is a key to the happiness of our life. Medicine takes
a big responsibility to take care of our health. Various supplements are available to meet the nutritional
requirement. One such supplement is a combination of piperine and coenzyme Q10. The medicines have
to be analyzed properly before introducing them to the market. As no analytical method is available for
simultaneous estimation of the combination, hence an attempt was made to develop a validated method
for the estimation of piperine and coenzyme Q10 simultaneously in bulk and its dosage form. A simple,
precise, accurate and validated reversed phase high performance liquid chromatography technique was
developed for the estimation of piperine and coenzyme Q10 in bulk and its tablet formulation. In this
developed method, Waters X Bridge C 18 column (250 mm×4.6 mm, 5 μm) was used as a stationary phase
and acetonitrile, tetrahydrofuran and water was used in 65:32:3 (v/v) ratio as mobile phase with 1 ml/min
flow rate. This isocratic separation was accomplished using Waters 2707 Autosampler high performance
liquid chromatography system, Waters 515 solvent delivery system, with photodiode array detector
detection at 275 nm. Chromatographic data was processed by Empower 2 software. The retention times
of coenzyme Q10 and piperine were 4.56 and 8.19 min respectively. The linearity ranges have lied between
4-6 μg/ml, 240-360 μg/ml for piperine and coenzyme Q10 respectively with 0.997 as correlation coefficient
for both.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-1235 |
Today people are focused on their health, as good health is a key to the happiness of our life. Medicine takes
a big responsibility to take care of our health. Various supplements are available to meet the nutritional
requirement. One such supplement is a combination of piperine and coenzyme Q10. The medicines have
to be analyzed properly before introducing them to the market. As no analytical method is available for
simultaneous estimation of the combination, hence an attempt was made to develop a validated method
for the estimation of piperine and coenzyme Q10 simultaneously in bulk and its dosage form. A simple,
precise, accurate and validated reversed phase high performance liquid chromatography technique was
developed for the estimation of piperine and coenzyme Q10 in bulk and its tablet formulation. In this
developed method, Waters X Bridge C 18 column (250 mm×4.6 mm, 5 μm) was used as a stationary phase
and acetonitrile, tetrahydrofuran and water was used in 65:32:3 (v/v) ratio as mobile phase with 1 ml/min
flow rate. This isocratic separation was accomplished using Waters 2707 Autosampler high performance
liquid chromatography system, Waters 515 solvent delivery system, with photodiode array detector
detection at 275 nm. Chromatographic data was processed by Empower 2 software. The retention times
of coenzyme Q10 and piperine were 4.56 and 8.19 min respectively. The linearity ranges have lied between
4-6 μg/ml, 240-360 μg/ml for piperine and coenzyme Q10 respectively with 0.997 as correlation coefficient
for both.
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