Histological assessment of fucoidan gelatine chitosan compound injectable hydrogel for bone regeneration in wistar rats
By: Eshwar, Shruthi
.
Contributor(s): Kranthi, K.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(6), Nov-Dec.Description: 1254-1260p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: To evaluate the effectiveness of fucoidan gelatine containing chitosan injectable hydrogel in bone
regeneration in rats by histopathological experimentation, a randomized laboratory experimental trial was
conducted on 30 Albino Wistar rats. Animals were randomly distributed into 3 groups. Micro defects of
2-3 mm were created in the tibia of each animal and the defects were treated with bone substitutes. Group 1
was treated with 1 % fucoidan-0.5 % chitosan hydrogel, Group 2 served as control and Group 3 was filled
with concentrated growth factor. All the animals were followed up for 28 d to appreciate wound healing
and new bone formation. Fisher’s exact test was used to study the difference in bone regeneration and
inflammation between the groups. The ratio of new bone per total area was significantly superior in both
test groups when compared to the control group at the end of 28 d. New osteoblasts were significantly more
in the fucoidan group followed by group 3 (p<0.05). No statistically significant difference in inflammation
status was observed between the 3 groups (p>0.05) at both 14 and 28 d. The presence of fucoidan in the
hydrogel significantly contributed to bone regeneration in animals thus proving to be a substitute for tissue
engineering.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-1249 |
To evaluate the effectiveness of fucoidan gelatine containing chitosan injectable hydrogel in bone
regeneration in rats by histopathological experimentation, a randomized laboratory experimental trial was
conducted on 30 Albino Wistar rats. Animals were randomly distributed into 3 groups. Micro defects of
2-3 mm were created in the tibia of each animal and the defects were treated with bone substitutes. Group 1
was treated with 1 % fucoidan-0.5 % chitosan hydrogel, Group 2 served as control and Group 3 was filled
with concentrated growth factor. All the animals were followed up for 28 d to appreciate wound healing
and new bone formation. Fisher’s exact test was used to study the difference in bone regeneration and
inflammation between the groups. The ratio of new bone per total area was significantly superior in both
test groups when compared to the control group at the end of 28 d. New osteoblasts were significantly more
in the fucoidan group followed by group 3 (p<0.05). No statistically significant difference in inflammation
status was observed between the 3 groups (p>0.05) at both 14 and 28 d. The presence of fucoidan in the
hydrogel significantly contributed to bone regeneration in animals thus proving to be a substitute for tissue
engineering.
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