Phytochemical and gas chromatography-mass spectrometry analysis of chenopodium album and stellaria media
By: Chak, Pooja
.
Contributor(s): Chaudhary, Deepti.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2021Edition: Vol.83(6), Nov-Dec.Description: 1261-1272p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: The current study was designed to conduct the physicochemical, phytochemical and gas chromatography-
mass spectrometry analysis of Chenopodium album and Stellaria media L. Methanolic extract of dried
leaves of Chenopodium album and Stellaria media was prepared and subsequently fractionated using
different solvents like hexane, chloroform, ethyl acetate. Obtained plant fractions were analyzed for the
presence of different secondary metabolites using phytochemical screening methods. Further, total phenolic
content, total flavonoid content and total alkaloid content in different plant fractions were also estimated
employing Ultraviolet-visible spectrophotometric methods. Results of phytochemical analysis revealed the
presence of different secondary metabolites including flavonoids, phenols, alkaloids, tannins, glycosides
and saponin. Further, gas chromatography-mass spectrometry analysis indicated presence of total 30 and
31 phytoconstituents in hexane and ethyl acetate fractions of methanolic extract of Chenopodium album
respectively whereas chloroform and ethyl acetate fractions of methanolic extract of Stellaria media showed
presence of total 33 and 35 phytoconstituents respectively. Gas chromatography-mass spectrometry
chromatogram showed abundance of polyphenolic compounds (85 % Phenol-2,4-bis(1,1-dimethylethyl),
phosphite (3:1) in hexane fractions of Chenopodium album, long-chain fatty acids (51 % hexadecanoic
acid, methyl ester in chloroform fractions of Chenopodium album), higher alkanes (66 % Octasiloxane-
1,1,3,3,5,5,7,7,9,9,11,11,13,13,15,15-hexadecamethyl in ethyl acetate fractions of Chenopodium album ).
Outcome of the study would certainly assist the investigators to explore these plants and to identify and
develop new leads for the treatment of different pathological conditions.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-1250 |
The current study was designed to conduct the physicochemical, phytochemical and gas chromatography-
mass spectrometry analysis of Chenopodium album and Stellaria media L. Methanolic extract of dried
leaves of Chenopodium album and Stellaria media was prepared and subsequently fractionated using
different solvents like hexane, chloroform, ethyl acetate. Obtained plant fractions were analyzed for the
presence of different secondary metabolites using phytochemical screening methods. Further, total phenolic
content, total flavonoid content and total alkaloid content in different plant fractions were also estimated
employing Ultraviolet-visible spectrophotometric methods. Results of phytochemical analysis revealed the
presence of different secondary metabolites including flavonoids, phenols, alkaloids, tannins, glycosides
and saponin. Further, gas chromatography-mass spectrometry analysis indicated presence of total 30 and
31 phytoconstituents in hexane and ethyl acetate fractions of methanolic extract of Chenopodium album
respectively whereas chloroform and ethyl acetate fractions of methanolic extract of Stellaria media showed
presence of total 33 and 35 phytoconstituents respectively. Gas chromatography-mass spectrometry
chromatogram showed abundance of polyphenolic compounds (85 % Phenol-2,4-bis(1,1-dimethylethyl),
phosphite (3:1) in hexane fractions of Chenopodium album, long-chain fatty acids (51 % hexadecanoic
acid, methyl ester in chloroform fractions of Chenopodium album), higher alkanes (66 % Octasiloxane-
1,1,3,3,5,5,7,7,9,9,11,11,13,13,15,15-hexadecamethyl in ethyl acetate fractions of Chenopodium album ).
Outcome of the study would certainly assist the investigators to explore these plants and to identify and
develop new leads for the treatment of different pathological conditions.
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