Binary solvent-assisted graphene synthesis by direct exfoliation of graphite powder and its cytotoxicity on siha cell line
By: Prabhat Kumar
.
Contributor(s): Jain, Purnima.
Publisher: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(3), Jul-Sep.Description: 732-739p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: A cost-effective mass production of pristine graphene nanosheet is still an important
challenge to harness its maximum potential in medical sciences. Here, we have used
a binary solvent mixture of low dielectric constant (1,2,4- trichlorobenzene,ε = 2.24
and benzylamine,ε = 4.6) to produce a high quantity (0.6 mg/ml) of graphene under
ultrasonication without compromising its quality. Obtained sample were characterized
through XRD, UV-Vis, Raman, FT-IR, TEM-SAED, and cytopotency was evaluated
on SiHa cell line. I G
/I 2D < 1.0 (~ 0.39) was achieved on more than 250 μm2 area,
indicating the uniformity and obtained I D /I G ratio was 1.21. No toxicity was found in the
case of 24 hr on the SiHa cell line. EC50 computation was 84.90 μg /ml for 48 hr and
62.03 μg /ml for 72 hr. Exfoliated graphene remained stable in binary solvent even after
1 year. The proposed experiment and obtained data can be powerful tools to explore
the synergic effect of binary solvent exfoliation in combination to establish graphene as
a biomaterial for neuronal network, tissue engineering, drug delivery, and regenerative
medicines, to name a few.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1380 |
A cost-effective mass production of pristine graphene nanosheet is still an important
challenge to harness its maximum potential in medical sciences. Here, we have used
a binary solvent mixture of low dielectric constant (1,2,4- trichlorobenzene,ε = 2.24
and benzylamine,ε = 4.6) to produce a high quantity (0.6 mg/ml) of graphene under
ultrasonication without compromising its quality. Obtained sample were characterized
through XRD, UV-Vis, Raman, FT-IR, TEM-SAED, and cytopotency was evaluated
on SiHa cell line. I G
/I 2D < 1.0 (~ 0.39) was achieved on more than 250 μm2 area,
indicating the uniformity and obtained I D /I G ratio was 1.21. No toxicity was found in the
case of 24 hr on the SiHa cell line. EC50 computation was 84.90 μg /ml for 48 hr and
62.03 μg /ml for 72 hr. Exfoliated graphene remained stable in binary solvent even after
1 year. The proposed experiment and obtained data can be powerful tools to explore
the synergic effect of binary solvent exfoliation in combination to establish graphene as
a biomaterial for neuronal network, tissue engineering, drug delivery, and regenerative
medicines, to name a few.
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