Evaluation of the cytotoxicity, apoptosis and autophagy induction by fucoidan in human hepatic adenocarcinoma cells
By: Dubey, Akanksha
.
Contributor(s): Sivaraman, Jayanthi.
Publisher: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(3), Jul-Sep.Description: 756-764p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Introduction: Seaweeds are the reservoir of bioactive constituents such as proteins,
polysaccharides, alkaloids, steroids and terpenoids that possess antioxidant, anti-
inflammatory and anti-cancerous properties.
Dictyota bartayresiana is a brown seaweed
with high antioxidant activity, making this species very valuable for human health.
Fucoidan is the sulphated polysaccharide obtained from brown algae
. Objectives: The
study aimed to extract, purify and characterize fucoidan from
Dictyota bartayresiana and
investigate its antioxidant and anti-cancer properties. Materials and Methods: Fucoidan
was isolated from
Dictyota bartayresiana and was investigated for its antioxidant property
by DPPH and ABTS assays, whereas cytotoxicity in SK-Hep-1 cells was checked using
MTT assay. The apoptosis assay was achieved via the Annexin-V-Apoptosis detection
kit, and images were obtained. To detect autophagy, western blotting was performed
for protein Atg5. Results: Fucoidan revealed positive radical scavenging actions even at
minor concentrations. The results acquired from the MTT assay revealed that cell death
initiated at minor concentrations of 0.781μg/ml and was in a dose-dependent mode.
The IC 50 value obtained was 30.96 (μg/ml). In our study, SK-Hep-1 cells revealed that
the intensities of the emission bands altered in response to apoptosis. It was observed
that after 24 hr of fucoidan treatment, the level of Atg5 increased with an increase in
fucoidan concentration and the band size observed for Atg5 was 55kDa. Conclusion:
Fucoidan possessed antioxidant and cytotoxic properties and was found helpful in
managing hepatocellular carcinoma.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1385 |
Introduction: Seaweeds are the reservoir of bioactive constituents such as proteins,
polysaccharides, alkaloids, steroids and terpenoids that possess antioxidant, anti-
inflammatory and anti-cancerous properties.
Dictyota bartayresiana is a brown seaweed
with high antioxidant activity, making this species very valuable for human health.
Fucoidan is the sulphated polysaccharide obtained from brown algae
. Objectives: The
study aimed to extract, purify and characterize fucoidan from
Dictyota bartayresiana and
investigate its antioxidant and anti-cancer properties. Materials and Methods: Fucoidan
was isolated from
Dictyota bartayresiana and was investigated for its antioxidant property
by DPPH and ABTS assays, whereas cytotoxicity in SK-Hep-1 cells was checked using
MTT assay. The apoptosis assay was achieved via the Annexin-V-Apoptosis detection
kit, and images were obtained. To detect autophagy, western blotting was performed
for protein Atg5. Results: Fucoidan revealed positive radical scavenging actions even at
minor concentrations. The results acquired from the MTT assay revealed that cell death
initiated at minor concentrations of 0.781μg/ml and was in a dose-dependent mode.
The IC 50 value obtained was 30.96 (μg/ml). In our study, SK-Hep-1 cells revealed that
the intensities of the emission bands altered in response to apoptosis. It was observed
that after 24 hr of fucoidan treatment, the level of Atg5 increased with an increase in
fucoidan concentration and the band size observed for Atg5 was 55kDa. Conclusion:
Fucoidan possessed antioxidant and cytotoxic properties and was found helpful in
managing hepatocellular carcinoma.
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