Carthamus tinctorius l. Inhibits proliferation of lung cancer A549 cells and tender’s mitochondrial protection
By: Dailah, Hamad Ghaleb
.
Publisher: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(3), Jul-Sep.Description: 789-794p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Background: Plant-based products are well-known as long-lasting chemo preventive and
chemotherapeutic medicines against cancer. Objectives: The purpose of this research
is to identify out how safflower extract affects A549 cells. Materials and Methods:
The antiproliferative activity was determined using the Trypan blue assay, while the
cytotoxicity was determined using the MTT assay. The ethidium bromide/acridine
orange (AO/EB) dual staining method was used to observe the apoptotic inducing effect
and the morphological assessment of A549 cells using phase contrast microscopy
was studied to discern the nuclear changes if any on treatment with safflower.
The mitochondrial membrane potential was studied using a cationic lipophilic dye
rhodamine 123 under a confocal microscope, and oxidative stress was assessed using
2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) labelling. Results: The trypan blue
assay speculated that there was a decrease in cell viability at highest concentration and
that the effect was dose dependent. The MTT assay revealed that cytotoxicity increases
in cells treated with higher concentration. The LC50 range of the sample was found at
250 μg/ml concentration at 52.2%, whereas the viability of the cell declined to 28.19%
at 350μg/ml concentration. The morphological features like shrinkage, detachment,
membrane blebbing, and distorted shape were observed on safflower treated A549 cells
that supports the antiproliferative activity. The ethidium bromide and acridine orange
staining substantiates that safflower extract induces apoptosis wherein the untreated
A549 cells showed green fluorescence with intact nuclear morphology and cells treated
with safflower extract (200 μg and 250 μg/ml), showed apoptotic bodies, clearly
validating the apoptosis inducing effect by safflower on A549 cells. Also, the safflower
extract protects the mitochondria by decreasing the oxidative stress and by altering the
mitochondrial membrane potential. Conclusion: Thus, it is found that safflower extract
exerts its antiproliferative activity by inducing apoptosis and protects mitochondria by
combating oxidative stress.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1389 |
Background: Plant-based products are well-known as long-lasting chemo preventive and
chemotherapeutic medicines against cancer. Objectives: The purpose of this research
is to identify out how safflower extract affects A549 cells. Materials and Methods:
The antiproliferative activity was determined using the Trypan blue assay, while the
cytotoxicity was determined using the MTT assay. The ethidium bromide/acridine
orange (AO/EB) dual staining method was used to observe the apoptotic inducing effect
and the morphological assessment of A549 cells using phase contrast microscopy
was studied to discern the nuclear changes if any on treatment with safflower.
The mitochondrial membrane potential was studied using a cationic lipophilic dye
rhodamine 123 under a confocal microscope, and oxidative stress was assessed using
2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA) labelling. Results: The trypan blue
assay speculated that there was a decrease in cell viability at highest concentration and
that the effect was dose dependent. The MTT assay revealed that cytotoxicity increases
in cells treated with higher concentration. The LC50 range of the sample was found at
250 μg/ml concentration at 52.2%, whereas the viability of the cell declined to 28.19%
at 350μg/ml concentration. The morphological features like shrinkage, detachment,
membrane blebbing, and distorted shape were observed on safflower treated A549 cells
that supports the antiproliferative activity. The ethidium bromide and acridine orange
staining substantiates that safflower extract induces apoptosis wherein the untreated
A549 cells showed green fluorescence with intact nuclear morphology and cells treated
with safflower extract (200 μg and 250 μg/ml), showed apoptotic bodies, clearly
validating the apoptosis inducing effect by safflower on A549 cells. Also, the safflower
extract protects the mitochondria by decreasing the oxidative stress and by altering the
mitochondrial membrane potential. Conclusion: Thus, it is found that safflower extract
exerts its antiproliferative activity by inducing apoptosis and protects mitochondria by
combating oxidative stress.
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