In vitro acetylcholinesterase inhibitory activity of different capsicum varieties by using chicken brain extract prepared by employing a home mixer-jar as an alternative to expensive tissue homogenizer
By: Pandy, Vijayapandi
.
Contributor(s): Shaik, Baji Rahamtulla.
Publisher: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(3) Jul-Aug.Description: 810-815p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Objectives: The present study aimed to investigate acetylcholinesterase (AchE) inhibitory
activity of methanolic extract of different capsicum varieties using isolated chicken’s
brain homogenate as an alternative for rodent’s brain homogenate by Ellman’s
in
vitro assay. In this study, we used a home mixer-jar as a substitute for the expensive
tissue homogenizer to mince the chicken brain along with conventional hand motor-
pestle homogenization technique. Materials and Methods: This
in vitro AchE inhibition
assay was performed as described by Ellman
et al. (1961) with minor modifications.
Results: The IC 50 values in ng/ml of the reference drug, neostigmine were found to be
149.92±37.72 and 347.22±81.50 using mortar and pestle (MP), and mixer-jar (MJ)
method of tissue homogenization techniques, respectively. The IC50 values in μg/ml of the
methanolic extract of different varieties of capsicum were found to be: red chilli by MP
method 1786.1±118.52; by MJ method 1858.5 ±136.79, green chilli by MP method
1418.8±94.16; by MJ method 1786.1±118.52and green capsicum (bell pepper) by
MP method 2022.8±244.5; by MJ method1813.3 ±45.63, respectively. Furthermore,
it was noted that the yield of AchE in brain extract prepared using a mixer jar was
high when compared with the brain homogenate prepared using the mortar and pestle.
Conclusion: The use of chicken brain and employing mixer-jar for brain homogenization
are evolved as good alternatives for unnecessary killing of laboratory animals and for
the brain tissue homogenization. All capsicum varieties (green chilli, red chilli, and green
capsicum) showed AchE inhibitory effect in
in vitro Ellman’s assay.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1392 |
Objectives: The present study aimed to investigate acetylcholinesterase (AchE) inhibitory
activity of methanolic extract of different capsicum varieties using isolated chicken’s
brain homogenate as an alternative for rodent’s brain homogenate by Ellman’s
in
vitro assay. In this study, we used a home mixer-jar as a substitute for the expensive
tissue homogenizer to mince the chicken brain along with conventional hand motor-
pestle homogenization technique. Materials and Methods: This
in vitro AchE inhibition
assay was performed as described by Ellman
et al. (1961) with minor modifications.
Results: The IC 50 values in ng/ml of the reference drug, neostigmine were found to be
149.92±37.72 and 347.22±81.50 using mortar and pestle (MP), and mixer-jar (MJ)
method of tissue homogenization techniques, respectively. The IC50 values in μg/ml of the
methanolic extract of different varieties of capsicum were found to be: red chilli by MP
method 1786.1±118.52; by MJ method 1858.5 ±136.79, green chilli by MP method
1418.8±94.16; by MJ method 1786.1±118.52and green capsicum (bell pepper) by
MP method 2022.8±244.5; by MJ method1813.3 ±45.63, respectively. Furthermore,
it was noted that the yield of AchE in brain extract prepared using a mixer jar was
high when compared with the brain homogenate prepared using the mortar and pestle.
Conclusion: The use of chicken brain and employing mixer-jar for brain homogenization
are evolved as good alternatives for unnecessary killing of laboratory animals and for
the brain tissue homogenization. All capsicum varieties (green chilli, red chilli, and green
capsicum) showed AchE inhibitory effect in
in vitro Ellman’s assay.
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