Estimation of antioxidant and cytotoxicity activities of extracts obtained from the leaves of folk medicinal plant benkara malabarica (lam)
By: Kalusalingam, Muthiah
.
Contributor(s): Balakrishnan, Veluchamy.
Publisher: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(3), Jul-Sep.Description: 838-845p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Objectives:
Benkara malabarica (Lam). is pharmacologically unexploited medicinal plant,
used in the folklore Paliyar in India. Medicinal plant systems to treat many ailments such
as cold, phlegm, stomach and body pains. The present investigation carried out with
objective of finding an alternative antioxidant and cytotoxicity medicines from traditionally
used medicinal plant and to provide ethnopharmacological information for its traditional
usage. Materials and Methods: Petroleum ether, chloroform and methanol were used
as solvents for the preparation of crude drug from the
B. malabarica leaves. DPPH and
O phenantroline reduction assay were used to estimate the antioxidant activities of the
extracts, while MTT assay was used to determine the cytotoxicity of extracts using Hep
G2 cell lines. Different concentration of extracts, in the range of 25, 50, 100, 150 and
200 μg/ml was used for the determination of antioxidant activities. In case of cytotoxicity
studies the extract concentration were 25, 50, 100, 200 and 500 μg/ml. Ascorbic acid
was used as standard in antioxidant studies and doxorubicin was employed as standard
in the cytotoxicity studies. Results: In both antioxidant and cytotoxicity studies, the
activities were increased with the increase the concentrations of extracts. The potential
of three extracts were in the following order, methanol, chloroform and petroleum ether
in both antioxidant and cytotoxicity studies. Conclusion: The remarkable antioxidant and
cytotoxicity activities, assayed through various
in vitro models in the present study. This
may be due to the presence of phytochemicals, such as phenol, flavonoid, alkaloid and
glycosides in the extracts. Scientific data generated from the present study supports the
usage of
B. malabarica in the folklore medicinal system.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1397 |
Objectives:
Benkara malabarica (Lam). is pharmacologically unexploited medicinal plant,
used in the folklore Paliyar in India. Medicinal plant systems to treat many ailments such
as cold, phlegm, stomach and body pains. The present investigation carried out with
objective of finding an alternative antioxidant and cytotoxicity medicines from traditionally
used medicinal plant and to provide ethnopharmacological information for its traditional
usage. Materials and Methods: Petroleum ether, chloroform and methanol were used
as solvents for the preparation of crude drug from the
B. malabarica leaves. DPPH and
O phenantroline reduction assay were used to estimate the antioxidant activities of the
extracts, while MTT assay was used to determine the cytotoxicity of extracts using Hep
G2 cell lines. Different concentration of extracts, in the range of 25, 50, 100, 150 and
200 μg/ml was used for the determination of antioxidant activities. In case of cytotoxicity
studies the extract concentration were 25, 50, 100, 200 and 500 μg/ml. Ascorbic acid
was used as standard in antioxidant studies and doxorubicin was employed as standard
in the cytotoxicity studies. Results: In both antioxidant and cytotoxicity studies, the
activities were increased with the increase the concentrations of extracts. The potential
of three extracts were in the following order, methanol, chloroform and petroleum ether
in both antioxidant and cytotoxicity studies. Conclusion: The remarkable antioxidant and
cytotoxicity activities, assayed through various
in vitro models in the present study. This
may be due to the presence of phytochemicals, such as phenol, flavonoid, alkaloid and
glycosides in the extracts. Scientific data generated from the present study supports the
usage of
B. malabarica in the folklore medicinal system.
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