Antidiabetic potential of ficus glomerata roots with a special emphasis on estimation of bioactive compounds by a novel validated HPTLC technique
By: Upadhye, Mohini
.
Contributor(s): Deokate, Uday.
Publisher: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(2), Apr-June.Description: 470-478p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Background: The data presented in this article for
Ficus glomerata Linn. belonging to
family Moraceace which is commonly found all over India. This study aimed towards
the development and validation of high-performance thin-layer chromatography (HPTLC)
method for simultaneous estimation of lupeol and quercetin from
Ficus glomerata and
correlate with its antidiabetic potential. Methods: The various fractions of ethanolic
extract of
Ficus glomerata root were prepared. The HPTLC analysis of quercetin and
lupeol which are the important phytoconstituents responsible for various pharmacological
actions was carried out at 525 nm. ICH guidelines were followed to validate this method
for accuracy, precision and repeatability. Results: The linearity range of quercetin and
lupeol were obtained as 400-2400 ng/spot and 1000- 5000 ng/spot respectively.
Percent drug content was highest in diethyl ether fraction (quercetin 2531.8 ng and
lupeol 1400 ng). The limit of detection value (LOD) obtained for quercetin and lupeol was
3.0793 and 3.1645 ng and the limit of quantification (LOQ) was 9.3314 and 9.5895 ng
respectively. This method developed was accurate, precise and simple has shown higher
resolution from other phytoconstituents present in the fractions. The method can be very
effectively applied for analyzing the quality of herbal material and formulations containing
Ficus glomerata. Antidiabetic activity of various fractions of ethanolic extract of
Ficus
glomerata roots was studied on alloxan-induced diabetic rats. Treatment with fractions
was continued for 11 days. The effect of the fractions on glucose was analyzed. Diabetic
rats treated with diethyl ether fraction exhibited a significant (
p<0.05) decrease in glucose
levels, indicating the potential use of
Ficus glomerata in diabetes mellitus. Conclusion:
As per the ICH guidelines, the HPTLC method used for simultaneous estimation of lupeol
and quercetin was accurate, precise and specific. The method used for phytochemical
standardization of various fractions of ethanolic extract of the roots of
Ficus glomerata
and correlated with its antidiabetic activity.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1432 |
Background: The data presented in this article for
Ficus glomerata Linn. belonging to
family Moraceace which is commonly found all over India. This study aimed towards
the development and validation of high-performance thin-layer chromatography (HPTLC)
method for simultaneous estimation of lupeol and quercetin from
Ficus glomerata and
correlate with its antidiabetic potential. Methods: The various fractions of ethanolic
extract of
Ficus glomerata root were prepared. The HPTLC analysis of quercetin and
lupeol which are the important phytoconstituents responsible for various pharmacological
actions was carried out at 525 nm. ICH guidelines were followed to validate this method
for accuracy, precision and repeatability. Results: The linearity range of quercetin and
lupeol were obtained as 400-2400 ng/spot and 1000- 5000 ng/spot respectively.
Percent drug content was highest in diethyl ether fraction (quercetin 2531.8 ng and
lupeol 1400 ng). The limit of detection value (LOD) obtained for quercetin and lupeol was
3.0793 and 3.1645 ng and the limit of quantification (LOQ) was 9.3314 and 9.5895 ng
respectively. This method developed was accurate, precise and simple has shown higher
resolution from other phytoconstituents present in the fractions. The method can be very
effectively applied for analyzing the quality of herbal material and formulations containing
Ficus glomerata. Antidiabetic activity of various fractions of ethanolic extract of
Ficus
glomerata roots was studied on alloxan-induced diabetic rats. Treatment with fractions
was continued for 11 days. The effect of the fractions on glucose was analyzed. Diabetic
rats treated with diethyl ether fraction exhibited a significant (
p<0.05) decrease in glucose
levels, indicating the potential use of
Ficus glomerata in diabetes mellitus. Conclusion:
As per the ICH guidelines, the HPTLC method used for simultaneous estimation of lupeol
and quercetin was accurate, precise and specific. The method used for phytochemical
standardization of various fractions of ethanolic extract of the roots of
Ficus glomerata
and correlated with its antidiabetic activity.
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