Anti-inflammatory and anti-hyperuricemic effect of ficus benghalensis bark extract in raw 246.7 cell line
By: Sabi, Essa M
.
Contributor(s): Mujamammi, Ahmed H.
Publisher: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(2), Apr-June.Description: 520-528p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Background: Hyperuricemia and inflammation are associated with the etiology and
pathogenesis of several metabolic diseases. Controlling the elevated levels of uric acid
and inflammation can be a potential therapeutic option for several metabolic diseases.
Aim: The current study examined the anti-inflammatory and anti-hyperuricemic properties
of
Ficus benghalensis bark extract
in vitro using murine macrophage RAW 246.7 cells.
Materials and Methods: Hydroalcoholic extracts of
Ficus benghalensis bark were obtained.
Cytotoxicity and cell viability test of the extracts was evaluated by MTT and Trypan blue
exclusion assay. Cells were microscopically observed for the presence of dead cells. To
explore the anti-inflammatory and anti-hyperuricemic activity, RAW 246.7 cells were
induced with lipopolysaccharide and treated at the concentrations of 20, 40, and 60 μg/ml
of
Ficus benghalensis bark extract and assessed for xanthine oxidase activity, uric acid
levels, reactive oxygen species, lipid peroxidation assay, nitric oxide levels and gene
expression studies of a pro-inflammatory and anti-inflammatory cytokine such as TNF-α
and IL- 10. Results: A dose-dependent reduction in the xanthine oxidase activity when
treated with the bark extract with higher inhibition at 60 μg/ml. Reduction in the amount
of uric acid, ROS, nitric oxide, and lipid peroxidation are similar to xanthine oxidase
activity with a significant decrease in all treated groups and higher reduction at 60 μg/
ml treatment. Gene expression study showed downregulation of TNF-α and upregulation
of IL- 10 in treated groups. Conclusion: Our study demonstrates that
Ficus benghalensis
bark extract exhibits anti-hyperuricemic and anti-inflammatory effects possibly through
xanthine oxidase inhibition. The results provide evidence that
Ficus benghalensis bark
extract may have the potential to treat hyperuricemia and complications related to
hyperuricemia.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1444 |
Background: Hyperuricemia and inflammation are associated with the etiology and
pathogenesis of several metabolic diseases. Controlling the elevated levels of uric acid
and inflammation can be a potential therapeutic option for several metabolic diseases.
Aim: The current study examined the anti-inflammatory and anti-hyperuricemic properties
of
Ficus benghalensis bark extract
in vitro using murine macrophage RAW 246.7 cells.
Materials and Methods: Hydroalcoholic extracts of
Ficus benghalensis bark were obtained.
Cytotoxicity and cell viability test of the extracts was evaluated by MTT and Trypan blue
exclusion assay. Cells were microscopically observed for the presence of dead cells. To
explore the anti-inflammatory and anti-hyperuricemic activity, RAW 246.7 cells were
induced with lipopolysaccharide and treated at the concentrations of 20, 40, and 60 μg/ml
of
Ficus benghalensis bark extract and assessed for xanthine oxidase activity, uric acid
levels, reactive oxygen species, lipid peroxidation assay, nitric oxide levels and gene
expression studies of a pro-inflammatory and anti-inflammatory cytokine such as TNF-α
and IL- 10. Results: A dose-dependent reduction in the xanthine oxidase activity when
treated with the bark extract with higher inhibition at 60 μg/ml. Reduction in the amount
of uric acid, ROS, nitric oxide, and lipid peroxidation are similar to xanthine oxidase
activity with a significant decrease in all treated groups and higher reduction at 60 μg/
ml treatment. Gene expression study showed downregulation of TNF-α and upregulation
of IL- 10 in treated groups. Conclusion: Our study demonstrates that
Ficus benghalensis
bark extract exhibits anti-hyperuricemic and anti-inflammatory effects possibly through
xanthine oxidase inhibition. The results provide evidence that
Ficus benghalensis bark
extract may have the potential to treat hyperuricemia and complications related to
hyperuricemia.
Click on an image to view it in the image viewer
There are no comments for this item.