Determination of nitazoxanide in biological matrices by LC-MS/MS technique: method development and validation
By: Abdul Saleem Mohammad
.
Contributor(s): Jayanthi, B.
Publisher: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(2), Apr-June.Description: 539-545p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Background: Determination of pharmaceuticals in the biological matrices is essential for
toxicologic and pharmacokinetic applications. The main objective of the current work
was to develop a bioanalytical method for quantifying nitazoxanide in biological samples
by LC-MS/MS. Materials and Methods: Chromatographic elution of nitazoxanide and
linagliptin were achieved on C18
-hypersil (5 μ, 50x4.6mm) stationary phase with mobile
phase consisting of acetonitrile and 0.1% HCOOH (75:15, v/v) processed at 0.8 ml/min
flow rate. Linagliptin was utilized as an internal standard (IS). Results: The method
was linear in the concentration levels of 0.53–21.2 ng/ml with more than 0.999
r2
values, consisting of 0.53 ng/ml as the lower limit of quantification (LLOQ). Analytes
were subjected to pretreatment by liquid-liquid extraction (LLE) procedure with average
extraction recovery findings within 99.93±5.04%. Method accuracy findings were
present in between 96.44% to 104.31%, and the assessed percentage relative standard
deviation findings for within and between run precision were ≤ 4.68%. Conclusion: The
developed LC-MS/MS procedure for quantitating nitazoxanide in the biological matrix
was suitable for routine analysis of patients’ blood samples for pharmacokinetic studies
and drug monitoring.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1446 |
Background: Determination of pharmaceuticals in the biological matrices is essential for
toxicologic and pharmacokinetic applications. The main objective of the current work
was to develop a bioanalytical method for quantifying nitazoxanide in biological samples
by LC-MS/MS. Materials and Methods: Chromatographic elution of nitazoxanide and
linagliptin were achieved on C18
-hypersil (5 μ, 50x4.6mm) stationary phase with mobile
phase consisting of acetonitrile and 0.1% HCOOH (75:15, v/v) processed at 0.8 ml/min
flow rate. Linagliptin was utilized as an internal standard (IS). Results: The method
was linear in the concentration levels of 0.53–21.2 ng/ml with more than 0.999
r2
values, consisting of 0.53 ng/ml as the lower limit of quantification (LLOQ). Analytes
were subjected to pretreatment by liquid-liquid extraction (LLE) procedure with average
extraction recovery findings within 99.93±5.04%. Method accuracy findings were
present in between 96.44% to 104.31%, and the assessed percentage relative standard
deviation findings for within and between run precision were ≤ 4.68%. Conclusion: The
developed LC-MS/MS procedure for quantitating nitazoxanide in the biological matrix
was suitable for routine analysis of patients’ blood samples for pharmacokinetic studies
and drug monitoring.
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