Development of fast, precise and selective RP-HPLC methods for identification of possible degradation products of ivermectin in isolated rat hepatocytes and in different pH media
By: Dineva, Alexandrina
.
Contributor(s): Angelov, Borislav.
Publisher: Karnataka Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(2), Apr-June.Description: 546-552p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Aim: A new stability indicating RP-HPLC based methods were developed to identify the
possible degradation products of Ivermectin in isolated rat hepatocytes and in different
pH media using a C18 RP column. Complete validation, including linearity, accuracy,
recovery, precision, robustness, stability, and peak purity, was performed. Materials
and Methods: HPLC system of UltiMateDionex 3000 DAD with LiChrosorb C18 Column
were used in this study. During the investigation, for the removal of interfering fraction
of isolated rat hepatocytes a purification procedure, consisting of protein precipitation
followed by double filtration was developed. Results: Method I was applied effectively
and the results indicated two major hepatic metabolites of Ivermectin. Method II was
developed to monitor the chemical stability in close to physiological conditions. The
appearance of a new peak and the proportional decrease in the sample concentration
indicate a liability in buffer with pH12. Conclusion: The obtained results demonstrated
that the degradation products of Ivermectin in hepatic cells and in alkali media are
different.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-1447 |
Aim: A new stability indicating RP-HPLC based methods were developed to identify the
possible degradation products of Ivermectin in isolated rat hepatocytes and in different
pH media using a C18 RP column. Complete validation, including linearity, accuracy,
recovery, precision, robustness, stability, and peak purity, was performed. Materials
and Methods: HPLC system of UltiMateDionex 3000 DAD with LiChrosorb C18 Column
were used in this study. During the investigation, for the removal of interfering fraction
of isolated rat hepatocytes a purification procedure, consisting of protein precipitation
followed by double filtration was developed. Results: Method I was applied effectively
and the results indicated two major hepatic metabolites of Ivermectin. Method II was
developed to monitor the chemical stability in close to physiological conditions. The
appearance of a new peak and the proportional decrease in the sample concentration
indicate a liability in buffer with pH12. Conclusion: The obtained results demonstrated
that the degradation products of Ivermectin in hepatic cells and in alkali media are
different.
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