Background: In general, use of herbal medicines are considered as the backbone in
traditional system of medicines as it has been used for food, flavoring agents and in the
form of medicines. Keeping in knowledge the increasing demand of herbal drugs over their
synthetic counter parts and to restrain the worldwide problem of multidrug resistance
(MDR) in hepatoprotective activity.
Neolamarckia cadamba that is commonly called
Cadamb is a standout amongst the most significant therapeutic plants having a place
with the Rubiaceae family. It is one of such Ayurvedic cure that has been referenced in
numerous Indian restorative written works. The arrangement of work includes gathering
and confirmation of stem bark of cadamb plant pursued by shade drying, granulating,
and extraction by two-fold cool maceration, phytochemical examination lastly screening
of pharmacological exercises as expressed. Aim: The present study was carried out to
establish hepatoprotective activity of hydro alcoholic extract of stem bark of
Neolamarckia
cadamba by using two established hepatotoxic screening models in Swiss albino
mice (chloroform induced hepatotoxicity and iron over dose induced hepatotoxicity).
Materials and Methods: The hepatoprotective activity of
Neolamarckia cadamba was
screened by hydro alcoholic extract of stem bark of
Neolamarckia cadamba (NCHAE).
The experimental animals were treated with corn oil and chloroform for a period of
7 days at a dose of 0.75mg/kg body weight, p.o to induce hepatotoxicity. In the second
experimental model over dose of iron dextran induced hepatic damage was performed
by administering 100 mg/kg, i.p.upto7 days, 3 consecutive periods. Blood samples were
collected for the estimation of biochemical parameters and histopathological changes
in liver was also performed. Silymarin (50 mg/kg; body weight) was used as standard
referral of hepatoprotective agent. Results: Chloroform and iron over dose treatment
were induced higher levels of liver marker enzymes and showed damage of hepatocytes.
In histopathological findings there were significant opposed treatment with
Neolamarckia
cadamba dose dependently. The results indicated that biochemical changes produced
by both the hepatotoxicity models were restored to normal by NCHAE. In the present
study, the results of NCHAE was found significant hepatoprotective effect (***
p<0.01)
against both the inducing agents, which was indicated by the enhancement in biochemical
parameters. Conclusion: The current study confirmed the hepatoprotective effect
of NCHAE against the chloroform induced hepatotoxicity and iron over dose induced
hepatotoxicity models and the activity is likely related to its potent antioxidant and iron-
chelating property. NCHAE therefore needs to be for further in-deep studies to correlate
its bioactive constituents responsible for the pharmacological activity selected.