Icariin regulates cell cycle related proteins and apoptosis related proteins and affects cell cycle and apoptosis of medulloblastoma cells
By: XU, C
.
Contributor(s): Xu, M.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(2), Mar-Apr.Description: 281-286p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: To investigate the effect of icariin on cell cycle and apoptosis of medulloblastoma cells and its mechanism.
Medulloblastoma cell line D341 was cultured and divided into control group, low dose group, medium
dose group and high dose group. The number and area of cell clones in low, medium and high dose groups
were significantly lower than those in control group, and decreased with the increase of dose (p<0.05). The
proportion of synthesis phase in the low, medium and high dose groups was significantly higher than that
in the control group and increased with the increase of dose, while the proportion of growth 1 phase in the
low, medium and high dose groups was significantly lower than that in the control group and decreased
with the increase of dose (p<0.05). The protein expressions of cyclin A, cyclin-dependent kinase 2 and
cyclin B1 in low, medium and high dose groups were significantly lower than those in control group and
decreased with the increase of dose (p<0.05). The apoptosis rate of low, medium and high dose groups
was significantly higher than that of control group and increased with the increase of dose (p<0.05). The
expression of Bcl-2-associated X protein in low, medium and high dose groups was significantly lower
than that in control group and decreased with the increase of dose. The expression of B-cell lymphoma 2,
cleaved caspase-3, cleaved caspase-9 and cleaved poly (ADP-ribose) polymerase protein in low, medium
and high dose groups was significantly higher than that in control group and increased with the increase
of dose (p<0.05). Icariin can inhibit the proliferation and colony forming ability of medulloblastoma cells,
induce cell arrest in synthesis phase by inhibiting the expression of cyclin A, cyclin-dependent kinase 2 and
cyclin B1, and induce cell apoptosis by regulating the expression of B-cell lymphoma 2, cleaved caspase-3,
cleaved caspase-9 and cleaved poly (ADP-ribose) polymerase protein.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
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Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-2169 |
To investigate the effect of icariin on cell cycle and apoptosis of medulloblastoma cells and its mechanism.
Medulloblastoma cell line D341 was cultured and divided into control group, low dose group, medium
dose group and high dose group. The number and area of cell clones in low, medium and high dose groups
were significantly lower than those in control group, and decreased with the increase of dose (p<0.05). The
proportion of synthesis phase in the low, medium and high dose groups was significantly higher than that
in the control group and increased with the increase of dose, while the proportion of growth 1 phase in the
low, medium and high dose groups was significantly lower than that in the control group and decreased
with the increase of dose (p<0.05). The protein expressions of cyclin A, cyclin-dependent kinase 2 and
cyclin B1 in low, medium and high dose groups were significantly lower than those in control group and
decreased with the increase of dose (p<0.05). The apoptosis rate of low, medium and high dose groups
was significantly higher than that of control group and increased with the increase of dose (p<0.05). The
expression of Bcl-2-associated X protein in low, medium and high dose groups was significantly lower
than that in control group and decreased with the increase of dose. The expression of B-cell lymphoma 2,
cleaved caspase-3, cleaved caspase-9 and cleaved poly (ADP-ribose) polymerase protein in low, medium
and high dose groups was significantly higher than that in control group and increased with the increase
of dose (p<0.05). Icariin can inhibit the proliferation and colony forming ability of medulloblastoma cells,
induce cell arrest in synthesis phase by inhibiting the expression of cyclin A, cyclin-dependent kinase 2 and
cyclin B1, and induce cell apoptosis by regulating the expression of B-cell lymphoma 2, cleaved caspase-3,
cleaved caspase-9 and cleaved poly (ADP-ribose) polymerase protein.
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