In vitro evaluation of anti-inflammatory and anti- cancer activities of the cocos nucifera flower extract and the phytochemical identification by gas chromatography/mass spectrometry analysis
By: Grace, V. M. Berlin
.
Contributor(s): Monisha, M.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(2), Mar-Apr.Description: 348-357p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: The flowers of Cocos nucifera (L.) have a traditional use as a healer of inflammatory disorders and are
available as Ayurveda formulations. But the scientific knowledge on the bioactive phytochemicals and
their medicinal effects are lacking. Hence, as a preliminary study, we have extracted the phytochemicals
from the flowers of Cocos nucifera(L.) using ethanol and subjected to biochemical tests as well as gas
chromatography–mass spectrometry analysis for phytochemical screening. The anti-cancer effect on
human lung cancer cell line was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide
assay for 25-200 μg/ml of the extract and the anti-inflammatory activity for different concentrations (1000-
8000 μg/ml) of the extract was evaluated using in vitro egg albumin degradation assay. The biochemical tests
have indicated the presence of phytosterols and tannins. A total of 152 phytochemicals were reported in the
gas chromatography–mass spectrometry analysis, which includes several phytosterols and few fatty acids,
polyphenols and terpenes. A dose dependent growth inhibitory action (96.15 % by 200 μg/ml) was exerted
on human lung cancer cell line cells by the extract (half-maximal inhibitory concentration value-90.2 μg/
ml). An effective inhibition on protein degradation (>50 %) as an indication of anti-inflammatory activity
was exerted by the extract from 1000 μg/ml and the 4000 μg/ml treatment has shown a similar action as the
500 μg/ml of standard anti-inflammatory drug, diclofenacsodium has shown. The secondary metabolites
such as quercetinderivative, eugenol, catechol, stigmasterol, campesterol, t-butylhydroquinone identified
might have exerted these medicinal effects. This study results thus gives a clear medicinal impact of the
ethanol extract of Cocos nucifera(L.) flower by showing a greater anti-inflammatory and anti-cancer
properties for further exploration of its pharmacological importance in the future.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2022-2176 |
The flowers of Cocos nucifera (L.) have a traditional use as a healer of inflammatory disorders and are
available as Ayurveda formulations. But the scientific knowledge on the bioactive phytochemicals and
their medicinal effects are lacking. Hence, as a preliminary study, we have extracted the phytochemicals
from the flowers of Cocos nucifera(L.) using ethanol and subjected to biochemical tests as well as gas
chromatography–mass spectrometry analysis for phytochemical screening. The anti-cancer effect on
human lung cancer cell line was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide
assay for 25-200 μg/ml of the extract and the anti-inflammatory activity for different concentrations (1000-
8000 μg/ml) of the extract was evaluated using in vitro egg albumin degradation assay. The biochemical tests
have indicated the presence of phytosterols and tannins. A total of 152 phytochemicals were reported in the
gas chromatography–mass spectrometry analysis, which includes several phytosterols and few fatty acids,
polyphenols and terpenes. A dose dependent growth inhibitory action (96.15 % by 200 μg/ml) was exerted
on human lung cancer cell line cells by the extract (half-maximal inhibitory concentration value-90.2 μg/
ml). An effective inhibition on protein degradation (>50 %) as an indication of anti-inflammatory activity
was exerted by the extract from 1000 μg/ml and the 4000 μg/ml treatment has shown a similar action as the
500 μg/ml of standard anti-inflammatory drug, diclofenacsodium has shown. The secondary metabolites
such as quercetinderivative, eugenol, catechol, stigmasterol, campesterol, t-butylhydroquinone identified
might have exerted these medicinal effects. This study results thus gives a clear medicinal impact of the
ethanol extract of Cocos nucifera(L.) flower by showing a greater anti-inflammatory and anti-cancer
properties for further exploration of its pharmacological importance in the future.
Click on an image to view it in the image viewer
There are no comments for this item.