Protective effect of polyherbal extract on gentamicin- induced renal injury in wistar rats
By: Solanki, Shivani
.
Contributor(s): Modi, C. M.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(2), Mar-Apr.Description: 369-379p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: This study was carried out to evaluate the protective effects of polyherbal extract against gentamicin
induced renal injury in rats. 36 male Wistar rats were divided into six groups. Gentamicin was administered
(100 mg/kg, intraperitoneally for 10 d) to all groups except normal control group. Polyherbal extract
was administered to three groups (gentamicin+polyherbal extract 100 mg/kg, gentamicin+polyherbal
extract 200 mg/kg and gentamicin+polyherbal extract 300 mg/kg) at 100, 200 and 300 mg/kg body weight
orally for 21 d, respectively. Taurine was taken as standard drug and given at the dose rate of 50 mg/
kg, orally for 21 d to positive group. The in vitro antioxidant activity was determined using 2,2-diphenyl-
1-picryl-hydrazyl-hydrate. The polyherbal extract was also subjected to phytochemical analysis using
liquid chromatography quadrupole time-of-flight mass spectrometry. Renal functions were assessed by
evaluating biochemical parameters and oxidative stress parameters. Histopathological examinations were
also conducted to confirm the renal damage. The treatment of polyherbal extract at dose of 300 mg/kg
body weight attenuated significantly (p<0.05) urea, creatinine and uric acid levels in gentamicin induced
nephrotoxicity model in rats. Similarly, activity of catalase in kidney of rats was found to have significant
(p<0.05) increase in polyherbal extract treatment at 300 mg/kg body weight. Insignificant altered level
of glutathione and superoxide dismutase activity was observed in kidney of rats in all treatment groups.
Renal histopathology also showed that polyherbal extract reduced the tubular degeneration, interstitial
infiltration of inflammatory cells and tubular necrosis. Liquid chromatography quadrupole time-of-flight
mass spectrometry analysis of polyherbal extract showed the presence of flavonoids, tannins, gallic acid
and tricarboxylic acid. The polyherbal extract at the dose of 200 μg/ml have shown the maximum radical
scavenging activity which indicated concentration dependent antioxidant activity. These results suggest
that polyherbal extract at dose of 300 mg/kg body weight, orally for 21 d exhibits a potent nephroprotective
effect on gentamicin-induced kidney damage in rats, which may be due to the increase in biochemical
parameters, antioxidant enzymes activity in rats).
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-2178 |
This study was carried out to evaluate the protective effects of polyherbal extract against gentamicin
induced renal injury in rats. 36 male Wistar rats were divided into six groups. Gentamicin was administered
(100 mg/kg, intraperitoneally for 10 d) to all groups except normal control group. Polyherbal extract
was administered to three groups (gentamicin+polyherbal extract 100 mg/kg, gentamicin+polyherbal
extract 200 mg/kg and gentamicin+polyherbal extract 300 mg/kg) at 100, 200 and 300 mg/kg body weight
orally for 21 d, respectively. Taurine was taken as standard drug and given at the dose rate of 50 mg/
kg, orally for 21 d to positive group. The in vitro antioxidant activity was determined using 2,2-diphenyl-
1-picryl-hydrazyl-hydrate. The polyherbal extract was also subjected to phytochemical analysis using
liquid chromatography quadrupole time-of-flight mass spectrometry. Renal functions were assessed by
evaluating biochemical parameters and oxidative stress parameters. Histopathological examinations were
also conducted to confirm the renal damage. The treatment of polyherbal extract at dose of 300 mg/kg
body weight attenuated significantly (p<0.05) urea, creatinine and uric acid levels in gentamicin induced
nephrotoxicity model in rats. Similarly, activity of catalase in kidney of rats was found to have significant
(p<0.05) increase in polyherbal extract treatment at 300 mg/kg body weight. Insignificant altered level
of glutathione and superoxide dismutase activity was observed in kidney of rats in all treatment groups.
Renal histopathology also showed that polyherbal extract reduced the tubular degeneration, interstitial
infiltration of inflammatory cells and tubular necrosis. Liquid chromatography quadrupole time-of-flight
mass spectrometry analysis of polyherbal extract showed the presence of flavonoids, tannins, gallic acid
and tricarboxylic acid. The polyherbal extract at the dose of 200 μg/ml have shown the maximum radical
scavenging activity which indicated concentration dependent antioxidant activity. These results suggest
that polyherbal extract at dose of 300 mg/kg body weight, orally for 21 d exhibits a potent nephroprotective
effect on gentamicin-induced kidney damage in rats, which may be due to the increase in biochemical
parameters, antioxidant enzymes activity in rats).
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