Protective effect of nigella sativa seeds extract against ethanol-induced gastric ulcer in rats: possible soluble epoxide hydrolase inhibition
By: Geetha, Kannoth Mukundan
.
Contributor(s): Kondareddypally, Nanjundappa Anitha.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(1), Jan-Feb.Description: 41-48p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: Nigella sativa seeds methanolic extract was prepared by a simple maceration process. The extract was
subjected to preliminary phytochemical screening and high-performance thin-layer chromatography
for linoleic and linolenic acid and tested for in vitro soluble epoxide hydrolase inhibition activity. Then
the extract (100, 150 and 200 mg/kg) was evaluated against ethanol-induced stomach ulcer in rats using
1-(1-propanoylpiperidin-4-yl)-3-(4-trifluoromethoxy)phenyl) urea (1 mg/kg) and omeprazole (20 mg/
kg) as reference standards. The plant extract contains flavonoids, alkaloids, proteins, free amino acids,
tannins, flavonoids and linoleic acid. The in vitro soluble epoxide hydrolase inhibition test results reveal
that Nigella sativa seeds methanolic extract inhibit human soluble epoxide hydrolase enzyme (half maximal
inhibitory concentration=2.619 μg/ml). The Nigella sativa seeds methanolic extract reversed macroscopic
and microscopic alteration and attenuated the levels of myeloperoxidase, tumour necrosis factor-alpha,
interleukin-6, 5-hydroxyeicosatetraenoic acid in ulcer rats. These results were comparable with standard
control. In conclusion, Nigella sativa seeds methanolic extract has a protective action against ethanol-
induced ulcers in rats.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
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Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-2217 |
Nigella sativa seeds methanolic extract was prepared by a simple maceration process. The extract was
subjected to preliminary phytochemical screening and high-performance thin-layer chromatography
for linoleic and linolenic acid and tested for in vitro soluble epoxide hydrolase inhibition activity. Then
the extract (100, 150 and 200 mg/kg) was evaluated against ethanol-induced stomach ulcer in rats using
1-(1-propanoylpiperidin-4-yl)-3-(4-trifluoromethoxy)phenyl) urea (1 mg/kg) and omeprazole (20 mg/
kg) as reference standards. The plant extract contains flavonoids, alkaloids, proteins, free amino acids,
tannins, flavonoids and linoleic acid. The in vitro soluble epoxide hydrolase inhibition test results reveal
that Nigella sativa seeds methanolic extract inhibit human soluble epoxide hydrolase enzyme (half maximal
inhibitory concentration=2.619 μg/ml). The Nigella sativa seeds methanolic extract reversed macroscopic
and microscopic alteration and attenuated the levels of myeloperoxidase, tumour necrosis factor-alpha,
interleukin-6, 5-hydroxyeicosatetraenoic acid in ulcer rats. These results were comparable with standard
control. In conclusion, Nigella sativa seeds methanolic extract has a protective action against ethanol-
induced ulcers in rats.
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