Effects of morinda officinalis polysaccharides on the proliferation and viability of osteoblasts in rats with osteoporosis
By: Qiaq, F
.
Contributor(s): Hanqing, Li.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(1), Jan-Feb.Description: 58-65p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: We aimed to evaluate the effects of Morinda officinalis polysaccharides on the proliferation and viability
of osteoblasts in rats with osteoporosis. 24 non-parous Sprague-Dawley rats were assigned into normal,
model, low-dose and high-dose Morinda officinalis polysaccharide groups. The model of osteoporosis
was established by ovariectomy. After successful modeling, 50 mg/kg and 75 mg/kg Morinda officinalis
polysaccharides were subcutaneously injected in low-dose and high-dose Morinda officinalis polysaccharide
groups, respectively. After 3 w of intervention, osteoblasts were prepared using the skull and their
proliferation index and alkaline phosphatase activity were measured. The histopathological changes in
femoral tissues were observed. The bone mineral density and levels of serum osteocalcin, calcium and
phosphorus, as well as the protein levels of receptor activator of nuclear factor kappa-B, receptor activator
of nuclear factor kappa-B ligand and osteoprotegerin were determined. The proliferation index and
alkaline phosphatase activity were highest in high-dose Morinda officinalis polysaccharide group (p<0.05).
Bone tissues had decreased cell spaces and gradually reduced spaces between the medullary cavities in
Morinda officinalis polysaccharide groups. Model group had the highest bone mineral density and levels of
serum osteocalcin, calcium and phosphorus (p<0.05). The protein expression levels of receptor activator of
nuclear factor kappa-B ligand and receptor activator of nuclear factor kappa-B showed a decreasing trend,
while that of osteoprotegerin displayed a rising trend sequentially in model, low-dose, high-dose Morinda
officinalis polysaccharide and normal groups (p<0.05). Morinda officinalis polysaccharides promotes the
proliferation of osteoblasts, enhances their viability and maintains the balance between bone resorption
and formation in rats with osteoporosis probably by modulating the protein expression of the receptor
activator of nuclear factor kappa-B ligand/receptor activator of nuclear factor kappa-B/osteoprotegerin
pathway.
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School of Pharmacy Archieval Section | Not for loan | 2022-2219 |
We aimed to evaluate the effects of Morinda officinalis polysaccharides on the proliferation and viability
of osteoblasts in rats with osteoporosis. 24 non-parous Sprague-Dawley rats were assigned into normal,
model, low-dose and high-dose Morinda officinalis polysaccharide groups. The model of osteoporosis
was established by ovariectomy. After successful modeling, 50 mg/kg and 75 mg/kg Morinda officinalis
polysaccharides were subcutaneously injected in low-dose and high-dose Morinda officinalis polysaccharide
groups, respectively. After 3 w of intervention, osteoblasts were prepared using the skull and their
proliferation index and alkaline phosphatase activity were measured. The histopathological changes in
femoral tissues were observed. The bone mineral density and levels of serum osteocalcin, calcium and
phosphorus, as well as the protein levels of receptor activator of nuclear factor kappa-B, receptor activator
of nuclear factor kappa-B ligand and osteoprotegerin were determined. The proliferation index and
alkaline phosphatase activity were highest in high-dose Morinda officinalis polysaccharide group (p<0.05).
Bone tissues had decreased cell spaces and gradually reduced spaces between the medullary cavities in
Morinda officinalis polysaccharide groups. Model group had the highest bone mineral density and levels of
serum osteocalcin, calcium and phosphorus (p<0.05). The protein expression levels of receptor activator of
nuclear factor kappa-B ligand and receptor activator of nuclear factor kappa-B showed a decreasing trend,
while that of osteoprotegerin displayed a rising trend sequentially in model, low-dose, high-dose Morinda
officinalis polysaccharide and normal groups (p<0.05). Morinda officinalis polysaccharides promotes the
proliferation of osteoblasts, enhances their viability and maintains the balance between bone resorption
and formation in rats with osteoporosis probably by modulating the protein expression of the receptor
activator of nuclear factor kappa-B ligand/receptor activator of nuclear factor kappa-B/osteoprotegerin
pathway.
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