Development of novel reverse phase high performance liquid chromatography method for simultaneous estimation of gallic acid, protocatechuic acid, vanillic acid and syringic acid in sugarcane roots
By: Jain,Vandana
.
Contributor(s): Prabhu, K. P.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(1), Jan-Feb.Description: 66-71p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: A novel, simple, accurate and precise isocratic high performance liquid chromatographic method was
developed for the estimation of four phenolic acids, namely gallic acid, protocatechuic acid, vanillic acid
and syringic acid in the sugarcane roots. The selected phenolic acids were analyzed using shim-pack high
performance liquid chromatography C18 column (250×4.6 mm, 5 μm) by isocratic elution. The mobile
phase used was methanol:water containing 0.2 % ortho-phosphoric acid in the ratio of 20:80 at 28°. The
flow rate was 1 ml/min and detection wavelength was set to 220 nm. The run time was kept 30 min. The
retention time of gallic acid, protocatechuic acid, vanillic acid and syringic acid was found to be 4.864,
8.741, 20.298 and 25.268 min, respectively. Linearity was established for all the selected phenolic acids in
the range of 0.25-20 μg/ml. The percentage recoveries of gallic acid, protocatechuic acid, vanillic acid and
syringic acid were found to be in the range of 98 %-102 %. The method was found to be robust. Therefore,
the method was successfully applied for the determination of the above mentioned phenolic acids in the
sugarcane rootss.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-2220 |
A novel, simple, accurate and precise isocratic high performance liquid chromatographic method was
developed for the estimation of four phenolic acids, namely gallic acid, protocatechuic acid, vanillic acid
and syringic acid in the sugarcane roots. The selected phenolic acids were analyzed using shim-pack high
performance liquid chromatography C18 column (250×4.6 mm, 5 μm) by isocratic elution. The mobile
phase used was methanol:water containing 0.2 % ortho-phosphoric acid in the ratio of 20:80 at 28°. The
flow rate was 1 ml/min and detection wavelength was set to 220 nm. The run time was kept 30 min. The
retention time of gallic acid, protocatechuic acid, vanillic acid and syringic acid was found to be 4.864,
8.741, 20.298 and 25.268 min, respectively. Linearity was established for all the selected phenolic acids in
the range of 0.25-20 μg/ml. The percentage recoveries of gallic acid, protocatechuic acid, vanillic acid and
syringic acid were found to be in the range of 98 %-102 %. The method was found to be robust. Therefore,
the method was successfully applied for the determination of the above mentioned phenolic acids in the
sugarcane rootss.
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