Zuogui pill on heat shock protein 70 and ultrastructure of brain neurons in senile dementia model rats
By: Qianqian, Li
.
Contributor(s): Xiaoli, Li.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(1), Jan-Feb.Description: 121-129p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: Alzheimer's disease is a pathological mechanism of chronic degenerative disease of brain function, which
has a profound influence on various brain diseases, with the highest incidence in the elderly population.
With the deepening of population aging, the incidence of Alzheimer's disease is also on the rise, seriously
affecting the quality of life of the elderly population. Therefore, the study of neuronal heat shock protein
70 and its ultrastructure is helpful to enhance the therapeutic effect and improve the quality of life of
patients. To observe the effect of Zuogui pill on heat shock protein and ultrastructure of brain neurons
in rats with Alzheimer's disease. The experimental rats were divided into model group, anti-cerebral
failure control group, Haboin control group, Zuogui pill low-dose group, Zuogui pill high-dose group
and sham operation group. The model group was injected with D-galactose intraperitoneal injection and
amyloid-beta injection into the hippocampus to prepare an Alzheimer's disease animal model. The two
control groups were injected with anti-Nao Shuai capsule and Haberman capsule while modeling. The
sham operation group was injected with the same amount of normal saline into the hippocampus of both
rats. After successful modeling, acetylcholinesterase of rat brain homogenate was detected by biochemical
method, expression of heat shock protein 70 in hippocampus of rats in each group was measured by reverse
transcription-polymerase chain reaction and ultrastructural changes of rat brain neurons were observed
by transmission electron microscope. Zuogui pill could inhibit acetylcholinesterase activity, reduce
apoptosis and stabilize the number of neurons. Ultrastructural observation also showed that hippocampal
neurons in each treatment group showed morphological characteristics different from the early apoptosis
in the model group. Zuogui pill has a positive effect on increasing the number of neurons surviving and
inhibiting cell death. It can effectively promote the repair of nerve cells in the brain, thus improving
memory impairment in Alzheimer's disease patients.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-2245 |
Alzheimer's disease is a pathological mechanism of chronic degenerative disease of brain function, which
has a profound influence on various brain diseases, with the highest incidence in the elderly population.
With the deepening of population aging, the incidence of Alzheimer's disease is also on the rise, seriously
affecting the quality of life of the elderly population. Therefore, the study of neuronal heat shock protein
70 and its ultrastructure is helpful to enhance the therapeutic effect and improve the quality of life of
patients. To observe the effect of Zuogui pill on heat shock protein and ultrastructure of brain neurons
in rats with Alzheimer's disease. The experimental rats were divided into model group, anti-cerebral
failure control group, Haboin control group, Zuogui pill low-dose group, Zuogui pill high-dose group
and sham operation group. The model group was injected with D-galactose intraperitoneal injection and
amyloid-beta injection into the hippocampus to prepare an Alzheimer's disease animal model. The two
control groups were injected with anti-Nao Shuai capsule and Haberman capsule while modeling. The
sham operation group was injected with the same amount of normal saline into the hippocampus of both
rats. After successful modeling, acetylcholinesterase of rat brain homogenate was detected by biochemical
method, expression of heat shock protein 70 in hippocampus of rats in each group was measured by reverse
transcription-polymerase chain reaction and ultrastructural changes of rat brain neurons were observed
by transmission electron microscope. Zuogui pill could inhibit acetylcholinesterase activity, reduce
apoptosis and stabilize the number of neurons. Ultrastructural observation also showed that hippocampal
neurons in each treatment group showed morphological characteristics different from the early apoptosis
in the model group. Zuogui pill has a positive effect on increasing the number of neurons surviving and
inhibiting cell death. It can effectively promote the repair of nerve cells in the brain, thus improving
memory impairment in Alzheimer's disease patients.
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