Crocodylus siamensis liver extracts exhibit antimicrobial activity and induce phagocytic activity in macrophage cells
By: Anwised, Preeyanan
.
Contributor(s): Suttee, Khantheeranut.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(1), Jan-Feb.Description: 197-206p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: Antimicrobial peptides are documented as having a crucial role in innate immune response. Many reports
have studied antimicrobial peptides from the crocodile. Thus, the antimicrobial activity of liver protein
extract from Crocodylus siamensis was examined. Our results show that P50 and P80, partially purified
crocodile liver protein extract at 45 mg/ml, can inhibit gram-positive bacteria with the highest percentages
of inhibition at 48.22 % and 37.54 % respectively, while gram-negative bacteria could be inhibited at
47.31 % and 35.32 %, respectively. However, only P80 was selected for further phagocytosis activity
investigation since it showed low cytotoxicity in the macrophage cell line. The results show that P80 can
kill bacteria by an intracellular killing process in phagocytic cells of macrophages in a dose-dependent
manner. Moreover, the internalization of phagocytosis activity of macrophages was assayed using
fluorescein isothiocyanate-labeled Escherichia coli and flow cytometry. The results reveal that P80 can
degrade bacteria by inducing phagosome formation in a time and dose-dependent manner (200-800 μg/ml).
In these findings, the liver protein extract of Crocodylus siamensis had an enhancing effect on macrophage
RAW 264.7 cells in the phagocytosis process.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-2254 |
Antimicrobial peptides are documented as having a crucial role in innate immune response. Many reports
have studied antimicrobial peptides from the crocodile. Thus, the antimicrobial activity of liver protein
extract from Crocodylus siamensis was examined. Our results show that P50 and P80, partially purified
crocodile liver protein extract at 45 mg/ml, can inhibit gram-positive bacteria with the highest percentages
of inhibition at 48.22 % and 37.54 % respectively, while gram-negative bacteria could be inhibited at
47.31 % and 35.32 %, respectively. However, only P80 was selected for further phagocytosis activity
investigation since it showed low cytotoxicity in the macrophage cell line. The results show that P80 can
kill bacteria by an intracellular killing process in phagocytic cells of macrophages in a dose-dependent
manner. Moreover, the internalization of phagocytosis activity of macrophages was assayed using
fluorescein isothiocyanate-labeled Escherichia coli and flow cytometry. The results reveal that P80 can
degrade bacteria by inducing phagosome formation in a time and dose-dependent manner (200-800 μg/ml).
In these findings, the liver protein extract of Crocodylus siamensis had an enhancing effect on macrophage
RAW 264.7 cells in the phagocytosis process.
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