Sulforaphane inhibits proliferation and apoptosis of colorectal cancer cells by down-regulating the cyclooxygenase-2/protein kinase B/glycogen synthase kinase-3 beta signaling pathway
By: Zhu, Y
.
Contributor(s): Hu, Haijun.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(1), Jan-Feb.Description: 219-223p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: To determine the effects of sulforaphane on the proliferation and apoptosis of colorectal cancer cell line
HCT116 and the levels of proteins related to the cyclooxygenase-2/protein kinase B/glycogen synthase
kinase-3 beta signal transduction. Colorectal cancer HCT116 cell lines were cultured in vitro and assigned
into sulforaphane (30, 60 and 120 μM) groups, simple cisplatin group and cisplatin+sulforaphane group.
The relative survival rate and apoptosis rate of HCT116 cells were determined by cell counting kit-8
assay and flow cytometry, respectively. Besides, the expression levels of proteins survivin, caspase-3,
cyclooxygenase-2, protein kinase B, phosphorylated-protein kinase B, glycogen synthase kinase-3 beta and
phosphorylated-glycogen synthase kinase-3 beta in HCT116 cells were measured through Western blotting.
Compared with that in control group, the relative survival rate of cells in 60 and 120 μM sulforaphane groups,
simple cisplatin group and cisplatin+sulforaphane group declined significantly (p<0.05). Sulforaphane can
exert its anti-tumor effect by inducing apoptosis and suppressing proliferation of HCT116 cells and its
mechanism may be associated with inhibition on the activation of the cyclooxygenase-2/protein kinase
B/glycogen synthase kinase-3 beta signaling pathway.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2022-2256 |
To determine the effects of sulforaphane on the proliferation and apoptosis of colorectal cancer cell line
HCT116 and the levels of proteins related to the cyclooxygenase-2/protein kinase B/glycogen synthase
kinase-3 beta signal transduction. Colorectal cancer HCT116 cell lines were cultured in vitro and assigned
into sulforaphane (30, 60 and 120 μM) groups, simple cisplatin group and cisplatin+sulforaphane group.
The relative survival rate and apoptosis rate of HCT116 cells were determined by cell counting kit-8
assay and flow cytometry, respectively. Besides, the expression levels of proteins survivin, caspase-3,
cyclooxygenase-2, protein kinase B, phosphorylated-protein kinase B, glycogen synthase kinase-3 beta and
phosphorylated-glycogen synthase kinase-3 beta in HCT116 cells were measured through Western blotting.
Compared with that in control group, the relative survival rate of cells in 60 and 120 μM sulforaphane groups,
simple cisplatin group and cisplatin+sulforaphane group declined significantly (p<0.05). Sulforaphane can
exert its anti-tumor effect by inducing apoptosis and suppressing proliferation of HCT116 cells and its
mechanism may be associated with inhibition on the activation of the cyclooxygenase-2/protein kinase
B/glycogen synthase kinase-3 beta signaling pathway.
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