Evaluation of anti-cancer activities of cranberries juice concentrate in osteosarcoma cell lines (MG-63)
By: Hattiholi, Aishwarya
.
Contributor(s): Tendulkar, Shivani.
Publisher: Bangalore Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(4), Oct-Dec.Description: 1141-1149p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Aim/Background: Osteosarcoma is one of the prevalent cancers occurring mostly in
adolescents and has a high risk of malignancy. With complications involved in the current
treatment strategies, alternates including the use of phytochemicals have gained fame.
Cranberries are known for their exceptional health benefits and have been explored for
their effective activities in various cancers. The current study aimed at evaluating the
anti-cancer properties of cranberry juice concentrate (CJC) on MG-63 cell line for human
osteosarcoma, by investigating its apoptotic activity through changes in cell viability and
mitochondrial membrane potential. Materials and Methods: Cranberry juice concentrate
was obtained by pulverization and lyophilization. The MG-63 cells were treated with
12.5-800 μg/mL of the CJC and incubated for 24, 48, and 72 hr. The percentage
cell viability and IC50 values were obtained. The mitochondrial membrane potential
and nuclear changes were examined. The induction of apoptosis was studied by flow
cytometer using BD cell Quest 7.5.3 software. GraphPad Prism was used for statistical
analysis with significant p-value at <0.05. Results: The IC50 values obtained for CJC
were 847.9, 637.4, and 440.6 μg/mL for 24, 48, and 72 hr respectively. Change in
the mitochondrial membrane potential and nuclear morphology was observed following
incubation with CJC. Flow cytometric analysis shows cells detected at early and late
apoptoic stages after treatment with CJC. Conclusion: Our result suggests that CJC has
significant effects on MG-63 osteosarcoma cells and can be considered to supplement
conventional therapeutic strategies.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2023-0112 |
Aim/Background: Osteosarcoma is one of the prevalent cancers occurring mostly in
adolescents and has a high risk of malignancy. With complications involved in the current
treatment strategies, alternates including the use of phytochemicals have gained fame.
Cranberries are known for their exceptional health benefits and have been explored for
their effective activities in various cancers. The current study aimed at evaluating the
anti-cancer properties of cranberry juice concentrate (CJC) on MG-63 cell line for human
osteosarcoma, by investigating its apoptotic activity through changes in cell viability and
mitochondrial membrane potential. Materials and Methods: Cranberry juice concentrate
was obtained by pulverization and lyophilization. The MG-63 cells were treated with
12.5-800 μg/mL of the CJC and incubated for 24, 48, and 72 hr. The percentage
cell viability and IC50 values were obtained. The mitochondrial membrane potential
and nuclear changes were examined. The induction of apoptosis was studied by flow
cytometer using BD cell Quest 7.5.3 software. GraphPad Prism was used for statistical
analysis with significant p-value at <0.05. Results: The IC50 values obtained for CJC
were 847.9, 637.4, and 440.6 μg/mL for 24, 48, and 72 hr respectively. Change in
the mitochondrial membrane potential and nuclear morphology was observed following
incubation with CJC. Flow cytometric analysis shows cells detected at early and late
apoptoic stages after treatment with CJC. Conclusion: Our result suggests that CJC has
significant effects on MG-63 osteosarcoma cells and can be considered to supplement
conventional therapeutic strategies.
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