Proteomic approach reveals the activation of apoptosis by padina gymnospora in oral cancer cell line
By: Yanyuan, Shi
.
Contributor(s): Zhaori, Getu.
Publisher: Bangalore Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(4), Oct-Dec.Description: 1150-1155p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Aim/Background: Cancer is still known to be a devastating disease condition. Even after
getting advancements in multimodality therapies, the success rate with respect to patient
survival is very limited. Hence there is an emerging need to find alternative medication
strategies for cancer treatment. Recently seaweeds are getting their significance due
to its tremendous medicinal properties. Among different category of seaweeds,
Padina
gymnospora which is a brown seaweed is known to possess many important bioactivities
including antimicrobial activity, anti-oxidative property and anti-inflammatory. Role of
this seaweed in treating oral cancer cells is not much explored in detail. Hence, this
study aims to understand the significance of
Padina gymnospora in oral cancer cell line
– SAS. Materials and Methods: The ethanol extract of Padina was treated to oral cancer
cell line – SAS and incubated for 24 hr. After incubation, MTT assay was performed
to find the efficiency of the extract. Further the cells were taken for protein extraction
and subjected to SDS PAGE, two-dimensional electrophoresis (2D) coupled with tandem
mass spectrometry approach. The differentially expressed proteins were further identified
and subjected to bioinformatic analysis. Results and Conclusion: It was observed that pro
apoptotic proteins including BIK, BAK1, BID, BAD and CASP8 were highly upregulated in
the Padina treated samples compared with untreated control samples. This data clearly
demonstrates that
Padina gymnospora induces the apoptosis in the oral cancer cells.
This study paves us the way to explore more about the therapeutic potential of
Padina
gymnospora in treating the oral cancer cells.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2023-0115 |
Aim/Background: Cancer is still known to be a devastating disease condition. Even after
getting advancements in multimodality therapies, the success rate with respect to patient
survival is very limited. Hence there is an emerging need to find alternative medication
strategies for cancer treatment. Recently seaweeds are getting their significance due
to its tremendous medicinal properties. Among different category of seaweeds,
Padina
gymnospora which is a brown seaweed is known to possess many important bioactivities
including antimicrobial activity, anti-oxidative property and anti-inflammatory. Role of
this seaweed in treating oral cancer cells is not much explored in detail. Hence, this
study aims to understand the significance of
Padina gymnospora in oral cancer cell line
– SAS. Materials and Methods: The ethanol extract of Padina was treated to oral cancer
cell line – SAS and incubated for 24 hr. After incubation, MTT assay was performed
to find the efficiency of the extract. Further the cells were taken for protein extraction
and subjected to SDS PAGE, two-dimensional electrophoresis (2D) coupled with tandem
mass spectrometry approach. The differentially expressed proteins were further identified
and subjected to bioinformatic analysis. Results and Conclusion: It was observed that pro
apoptotic proteins including BIK, BAK1, BID, BAD and CASP8 were highly upregulated in
the Padina treated samples compared with untreated control samples. This data clearly
demonstrates that
Padina gymnospora induces the apoptosis in the oral cancer cells.
This study paves us the way to explore more about the therapeutic potential of
Padina
gymnospora in treating the oral cancer cells.
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