Evaluation of the thrombolytic and antioxidant activity of leaf extracts of plumbago zeylanica l.
By: Guguloth, Sarvan Kumar
.
Contributor(s): Malothu, Narender.
Publisher: Bangalore Association of Pharmaceutical Teachers of India (APTI) 2022Edition: Vol.56(4), Oct-Dec.Description: 1181-1189p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical education and researchSummary: Background:
Plumbago zeylanica L. is one of the extremely accessible conventionally
used herbal plants with various biological activities. However, actions of
P. zeylanica
L on blood clotting and other complications of blood were indisposed therapeutically
studied. Therefore, the scope of the current exploration is to screen the thrombolytic,
antioxidant, and cytotoxic effects of leaf extracts. Materials and Methods: Thrombolytic
activity (
in vitro) was assessed with clot lysis and thrombin inhibitory ability. Further,
thrombolytic activity (
in vivo) was evaluated by a thrombotic tail (carrageenan-induced)
animal model. DPPH and nitric oxide (free radical) scavenging methods were employed
to check the
in vitro antioxidant property. Further cytotoxicity and acute oral toxicity
were assessed for plant extract. Results: The quantitative analysis elicits the presence
of the magnificent amount of the total phenolic content (96.8±7.92 mg GAE/g) and
total flavonoid content (63.52±4.54 mg QE/g) on the dry weight basis. The maximum
clot lysis (96.83%±0.657) of methanolic leaf extract was detected in
in vitro model at
800 μg/mL in 72 hr. A strong thrombin inhibition (94.63±2.12%) effect was observed
for methanol leaf extract at 2 mg/mL. In
in vivo studies a significant (
p< 0.001) clot
lysis was achieved at the tested dose (100, 200 and 300 mg/kg). DPPH radical and
nitric oxide scavenging activity showed the IC50 value of 25.47±0.51 and 56.32±0.85,
respectively. The methanolic extract was found safer up to the highest lethal dose of
2000 mg/kg. Conclusion: These findings suggested that the plant leaves are comprised
of significant thrombolytic properties. It could be a promising source for the existence of
antioxidant and thrombolytic agents.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2023-0121 |
Background:
Plumbago zeylanica L. is one of the extremely accessible conventionally
used herbal plants with various biological activities. However, actions of
P. zeylanica
L on blood clotting and other complications of blood were indisposed therapeutically
studied. Therefore, the scope of the current exploration is to screen the thrombolytic,
antioxidant, and cytotoxic effects of leaf extracts. Materials and Methods: Thrombolytic
activity (
in vitro) was assessed with clot lysis and thrombin inhibitory ability. Further,
thrombolytic activity (
in vivo) was evaluated by a thrombotic tail (carrageenan-induced)
animal model. DPPH and nitric oxide (free radical) scavenging methods were employed
to check the
in vitro antioxidant property. Further cytotoxicity and acute oral toxicity
were assessed for plant extract. Results: The quantitative analysis elicits the presence
of the magnificent amount of the total phenolic content (96.8±7.92 mg GAE/g) and
total flavonoid content (63.52±4.54 mg QE/g) on the dry weight basis. The maximum
clot lysis (96.83%±0.657) of methanolic leaf extract was detected in
in vitro model at
800 μg/mL in 72 hr. A strong thrombin inhibition (94.63±2.12%) effect was observed
for methanol leaf extract at 2 mg/mL. In
in vivo studies a significant (
p< 0.001) clot
lysis was achieved at the tested dose (100, 200 and 300 mg/kg). DPPH radical and
nitric oxide scavenging activity showed the IC50 value of 25.47±0.51 and 56.32±0.85,
respectively. The methanolic extract was found safer up to the highest lethal dose of
2000 mg/kg. Conclusion: These findings suggested that the plant leaves are comprised
of significant thrombolytic properties. It could be a promising source for the existence of
antioxidant and thrombolytic agents.
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