In vitroandin vivoscreening ofanti-inflammatory activity of methanolic and aqueous extracts of anogeissus latifolialeaves
By: Shaikh, Saba
.
Contributor(s): Ajaz Ahmed.
Publisher: M P Innovare Academic Sciences Pvt Ltd 2022Edition: Vol.14(11).Description: 65-72p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
International journal of pharmacy and pharmaceutical scienceSummary: Objective: To evaluate and compare anti-oxidant and anti-inflammatory activity of methanolic and aqueous extract of Anogeissus latifolialeavesMethods: The in vitroantioxidant activity was investigated usingnitric oxide radical inhibition activity assay, hydroxyl radical scavenging activity assay, DPPH free radical scavenging assay,and reducing power assay. The in vitroanti-inflammatory activity was investigated using erythrocyte membrane stabilization, inhibition of protein denaturation,and proteinase inhibitory activity the in vivoanti-inflammatory activity wasinvestigated using carrageenan-induced rat paw edema.The biochemical parameters wereevaluated inthe blood,which includedthe determination of serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminasevaluesand intheliver,which includes the estimation of lipid peroxidation, reduced glutathione,and superoxide dismutase. Results: The methanolic extract causedasignificant dose dependedonthe reduction of inflammation when compared withtheaqueous extract of Anogeissus latifolia.The anti-inflammatory activity of all groups was found to be comparable tothestandard indomethacin group.The maximum percent inhibition in paw edema was found in methanolic extract of Anogeisuss latifoliaatadose of 500 mg/kgwas 53.33%,with significant anti-inflammatory activity p<0.001.Conclusion: The leaf extract of Anogeissus latifolia possesses anti-oxidant and anti-inflammatory activity.The therapeutic effect of Anogeisuss latifolia extracts will encourage its use in the treatment of inflammation.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
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Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2023-0263 |
Objective: To evaluate and compare anti-oxidant and anti-inflammatory activity of methanolic and aqueous extract of Anogeissus latifolialeavesMethods: The in vitroantioxidant activity was investigated usingnitric oxide radical inhibition activity assay, hydroxyl radical scavenging activity assay, DPPH free radical scavenging assay,and reducing power assay. The in vitroanti-inflammatory activity was investigated using erythrocyte membrane stabilization, inhibition of protein denaturation,and proteinase inhibitory activity the in vivoanti-inflammatory activity wasinvestigated using carrageenan-induced rat paw edema.The biochemical parameters wereevaluated inthe blood,which includedthe determination of serum glutamic oxaloacetic transaminase and serum glutamic pyruvic transaminasevaluesand intheliver,which includes the estimation of lipid peroxidation, reduced glutathione,and superoxide dismutase. Results: The methanolic extract causedasignificant dose dependedonthe reduction of inflammation when compared withtheaqueous extract of Anogeissus latifolia.The anti-inflammatory activity of all groups was found to be comparable tothestandard indomethacin group.The maximum percent inhibition in paw edema was found in methanolic extract of Anogeisuss latifoliaatadose of 500 mg/kgwas 53.33%,with significant anti-inflammatory activity p<0.001.Conclusion: The leaf extract of Anogeissus latifolia possesses anti-oxidant and anti-inflammatory activity.The therapeutic effect of Anogeisuss latifolia extracts will encourage its use in the treatment of inflammation.
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