Phenolic composition, in vitro alpha-amylase and pancreatic lipase inhibitory effects, anti-inflammatory and antioxidant activities of ephedra altissima
By: Bouafia, W
.
Contributor(s): Hamdi, Amel.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(4), Jul-Aug.Description: 890-901p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: This study aimed to investigate the phytochemical composition, alpha-amylase and pancreatic lipase
inhibitory activities and anti-inflammatory and antioxidant properties of the crude extracts (petroleum
ether, ethyl acetate and n-butanol) obtained from the aerial parts of Ephedra altissima. The characterization
of the phenolic compounds in crude extracts was performed by high performance liquid chromatography-
photodiode array detection-electrospray ionization-mass spectrometry and the in vitro alpha-amylase and
pancreatic lipase inhibitory activities were evaluated using starch and p-nitrophenyl butyrate as substrates,
respectively. Furthermore, the anti-inflammatory activity was carried out by bovine serum albumin
denaturation method and the antioxidant capacity was assessed using five different assays. Flavonol and
flavone glycoside derivatives and phenolic acids, especially isovitexin-2-O-rhamnoside and vicenin II were
the major compounds in the n-butanol extract. Otherwise, the ethyl acetate extract presented kaempferol-3-
O-rhamnoside, quercetin-3-O-rhamnoside and protocatechuic acid as its main compounds. The n-butanol
extract revealed the highest content of total phenolic compounds. The results for antioxidant activity
showed that ethyl acetate extract possesses the strongest activity in all the tested methods. Moreover, the
ethyl acetate extract displayed the best anti-inflammatory, alpha-amylase and pancreatic lipase inhibitory
activities with half-maximal inhibitory concentration values of 126.43, 9.02 and 289.11 μg/ml, respectively.
These results give obvious support to the use of Ephedra altissima in traditional medicine for the treatment
of diabetic diseases. Also, the moderate alpha-amylase inhibitory activity of Ephedra altissima extracts
make advantage of its therapeutic applications compared to the synthetic drugs, since their strong alpha-
amylase inhibitory effects are associated to many digestive adverse effects including the abnormal bacterial
fermentation in the colon.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2023-0846 |
This study aimed to investigate the phytochemical composition, alpha-amylase and pancreatic lipase
inhibitory activities and anti-inflammatory and antioxidant properties of the crude extracts (petroleum
ether, ethyl acetate and n-butanol) obtained from the aerial parts of Ephedra altissima. The characterization
of the phenolic compounds in crude extracts was performed by high performance liquid chromatography-
photodiode array detection-electrospray ionization-mass spectrometry and the in vitro alpha-amylase and
pancreatic lipase inhibitory activities were evaluated using starch and p-nitrophenyl butyrate as substrates,
respectively. Furthermore, the anti-inflammatory activity was carried out by bovine serum albumin
denaturation method and the antioxidant capacity was assessed using five different assays. Flavonol and
flavone glycoside derivatives and phenolic acids, especially isovitexin-2-O-rhamnoside and vicenin II were
the major compounds in the n-butanol extract. Otherwise, the ethyl acetate extract presented kaempferol-3-
O-rhamnoside, quercetin-3-O-rhamnoside and protocatechuic acid as its main compounds. The n-butanol
extract revealed the highest content of total phenolic compounds. The results for antioxidant activity
showed that ethyl acetate extract possesses the strongest activity in all the tested methods. Moreover, the
ethyl acetate extract displayed the best anti-inflammatory, alpha-amylase and pancreatic lipase inhibitory
activities with half-maximal inhibitory concentration values of 126.43, 9.02 and 289.11 μg/ml, respectively.
These results give obvious support to the use of Ephedra altissima in traditional medicine for the treatment
of diabetic diseases. Also, the moderate alpha-amylase inhibitory activity of Ephedra altissima extracts
make advantage of its therapeutic applications compared to the synthetic drugs, since their strong alpha-
amylase inhibitory effects are associated to many digestive adverse effects including the abnormal bacterial
fermentation in the colon.
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