Normal view MARC view ISBD view

Formation of gefitinib-resistant lung cancer cell lines and their changes in epidermal growth factor receptor signaling pathway

By: Yunxi, Song.
Contributor(s): Jianxin, Ma.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(5), Sep-Oct.Description: 1269-1278p.Subject(s): PHARMACEUTICSOnline resources: Click here In: Indian journal of pharmaceutical sciencesSummary: Lung cancer is one of the top ten malignant tumors, seriously endangering people’s lives and health, with morbidity and mortality ranking first. Molecular targeted therapy overcomes the shortcomings of traditional chemotherapy. In recent years, the application of tumor therapy has become wider and wider. Gefitinib is the most successful molecular targeted drug for the treatment of lung cancer in recent years. The purpose of this article is to explore the establishment method of gefitinib-resistant lung cancer cell lines and the mechanism of epidermal growth factor receptor signaling pathway change. The method of gradually increasing the concentration of gefitinib was used to successfully establish NC-H1985 gefitinib- resistant cells. The strains were also analyzed for epidermal growth factor receptor, messenger ribonucleic acid expression at different times. The results show that this cell line has a certain difference compared to the parental NC-H1985 cell. With the extension of the culture time, the ribonucleic acid concentration decreased by 51 % and the 18S ribosomal ribonucleic acid, cycle threshold value increased by 39 %. In addition, the higher the relative expression of messenger ribonucleic acid in gefitinib-resistant lung cancer cells, the epidermal growth factor receptor signaling pathway is likely to change. From 24 h to 72 h, the relative expression of messenger ribonucleic acid has increased by 37.5 %. After gefitinib resistance culture of NC-H1985 gefitinib-resistant cells were 44.1 % in resting phase, 36.9 % in growth 1 phase, 11.4 % in synthesis phase, 4.3 % in growth 2 phase and 3.3 % in mitotic phase.
Tags from this library: No tags from this library for this title. Log in to add tags.
    average rating: 0.0 (0 votes)
Item type Current location Call number Status Date due Barcode Item holds
Articles Abstract Database Articles Abstract Database School of Pharmacy
Archieval Section
Not for loan 2023-1139
Total holds: 0

Lung cancer is one of the top ten malignant tumors, seriously endangering people’s lives and health,
with morbidity and mortality ranking first. Molecular targeted therapy overcomes the shortcomings of
traditional chemotherapy. In recent years, the application of tumor therapy has become wider and wider.
Gefitinib is the most successful molecular targeted drug for the treatment of lung cancer in recent years.
The purpose of this article is to explore the establishment method of gefitinib-resistant lung cancer cell
lines and the mechanism of epidermal growth factor receptor signaling pathway change. The method of
gradually increasing the concentration of gefitinib was used to successfully establish NC-H1985 gefitinib-
resistant cells. The strains were also analyzed for epidermal growth factor receptor, messenger ribonucleic
acid expression at different times. The results show that this cell line has a certain difference compared
to the parental NC-H1985 cell. With the extension of the culture time, the ribonucleic acid concentration
decreased by 51 % and the 18S ribosomal ribonucleic acid, cycle threshold value increased by 39 %.
In addition, the higher the relative expression of messenger ribonucleic acid in gefitinib-resistant lung
cancer cells, the epidermal growth factor receptor signaling pathway is likely to change. From 24 h to 72
h, the relative expression of messenger ribonucleic acid has increased by 37.5 %. After gefitinib resistance
culture of NC-H1985 gefitinib-resistant cells were 44.1 % in resting phase, 36.9 % in growth 1 phase, 11.4
% in synthesis phase, 4.3 % in growth 2 phase and 3.3 % in mitotic phase.

There are no comments for this item.

Log in to your account to post a comment.

Click on an image to view it in the image viewer

Unique Visitors hit counter Total Page Views free counter
Implemented and Maintained by AIKTC-KRRC (Central Library).
For any Suggestions/Query Contact to library or Email: librarian@aiktc.ac.in | Ph:+91 22 27481247
Website/OPAC best viewed in Mozilla Browser in 1366X768 Resolution.

Powered by Koha