Development and validation of a novel high performance thin layer chromatography method for simultaneous estimation of mangiferin and berberine in an ayurvedic medicine
By: Mokal, Ruchira
.
Contributor(s): Jadhav, Aruna.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2022Edition: Vol.84(5), Sep-Oct.Description: 1338-1342p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: Setebid tablet is the natural antihyperglycemic agent with powerful antioxidant action. A high-
performance thin layer chromatography method was developed for simultaneous estimation of mangiferin
and berberine from setebid tablets. Camag Linomat 5 sample applicator was used for application of
sample. Chromatographic separation of drugs was performed over thin layered chromatography plates
pre-coated with silica gel 60F254 using toluene:acetone:formic acid (3.5:5.5:1 v/v/v) as the mobile phase
via a linear ascending technique in twin trough chamber. Detection and quantification were carried out
at an iso-absorptive wavelength of 261 nm using thin layered chromatography scanner III. This method
showed good resolution for both drugs with retention factor 0.39±0.03 and 0.80±0.03 for mangiferin and
berberine, respectively. The calibration curves were linear in the range of 400-800 and 100-500 ng/spot
for mangiferin and berberine and correlation coefficients (r2) were 0.9995 and 0.9991, respectively. The
method was validated according to the international conference on harmonisation Q2 R1 guidelines for
accuracy, precision, limit of detection, linearity, limit of quantification, robustness and specificity. The
limits of detection were 26.18 and 18.50 ng/spot for mangiferin and berberine, respectively. In conclusion,
the developed method was rapid, simple, reliable and specific for the identification and quantification of
mangiferin and berberine.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
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School of Pharmacy Archieval Section | Not for loan | 2023-1148 |
Setebid tablet is the natural antihyperglycemic agent with powerful antioxidant action. A high-
performance thin layer chromatography method was developed for simultaneous estimation of mangiferin
and berberine from setebid tablets. Camag Linomat 5 sample applicator was used for application of
sample. Chromatographic separation of drugs was performed over thin layered chromatography plates
pre-coated with silica gel 60F254 using toluene:acetone:formic acid (3.5:5.5:1 v/v/v) as the mobile phase
via a linear ascending technique in twin trough chamber. Detection and quantification were carried out
at an iso-absorptive wavelength of 261 nm using thin layered chromatography scanner III. This method
showed good resolution for both drugs with retention factor 0.39±0.03 and 0.80±0.03 for mangiferin and
berberine, respectively. The calibration curves were linear in the range of 400-800 and 100-500 ng/spot
for mangiferin and berberine and correlation coefficients (r2) were 0.9995 and 0.9991, respectively. The
method was validated according to the international conference on harmonisation Q2 R1 guidelines for
accuracy, precision, limit of detection, linearity, limit of quantification, robustness and specificity. The
limits of detection were 26.18 and 18.50 ng/spot for mangiferin and berberine, respectively. In conclusion,
the developed method was rapid, simple, reliable and specific for the identification and quantification of
mangiferin and berberine.
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