Effect and association of circ_0084927 and microRNA-634 on lung cancer cell sensitivity to cisplatin
By: Gao, Yang
.
Contributor(s): Wang, Rui.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2023Edition: Vol.85(4), Jul-Aug.Description: 1118-1125p.Subject(s): PHARMACEUTICSOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: To investigate the role of circ_0084927 in lung cancer cell sensitivity to cisplatin. Half-maximal inhibitory
concentration value of cisplatin was detected by cell counting kit-8. circ_0084927 and microRNA-634
expression were determined by reverse transcription quantitative polymerase chain reaction. Cell
counting kit-8, scratch healing, colony formation as well as Transwell experiments were used for
analyzing cell viability, scratch healing rate, clone number and invasive ability, respectively. Dual-
luciferase reporter method was utilized to validate targeting relationship. The half-maximal inhibitory
concentration value of cisplatin and circ_0084927 expression were increased in A549/DDP cells, whereas
microRNA-634 expression displayed an opposite result in comparison with A549 cells (p<0.05). Relative
to the DDP+si-NC group, the cell optical density value, scratch healing rate, clone numbers and invasion
numbers were significantly reduced in the DDP+si-circ_0084927 group (p<0.05). Optical density value,
scratch healing rate, clone numbers as well as invasion numbers in the DDP+microRNA-634 group were
significantly reduced relative to the DDP+microRNA-NC group (p<0.05). Circ_0084927 directly bound
to microRNA-634. Relative to the DDP+si-circ_0084927+anti-microRNA-NC group, optical density
value, scratch healing rate, clone numbers as well as invasion numbers in the DDP+si-circ_0084927+anti-
microRNA-634 group were increased (p<0.05). Interference with circ_0084927 reduced the cisplatin
resistance of lung cancer cells by promoting microRNA-634.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2024-0422 |
To investigate the role of circ_0084927 in lung cancer cell sensitivity to cisplatin. Half-maximal inhibitory
concentration value of cisplatin was detected by cell counting kit-8. circ_0084927 and microRNA-634
expression were determined by reverse transcription quantitative polymerase chain reaction. Cell
counting kit-8, scratch healing, colony formation as well as Transwell experiments were used for
analyzing cell viability, scratch healing rate, clone number and invasive ability, respectively. Dual-
luciferase reporter method was utilized to validate targeting relationship. The half-maximal inhibitory
concentration value of cisplatin and circ_0084927 expression were increased in A549/DDP cells, whereas
microRNA-634 expression displayed an opposite result in comparison with A549 cells (p<0.05). Relative
to the DDP+si-NC group, the cell optical density value, scratch healing rate, clone numbers and invasion
numbers were significantly reduced in the DDP+si-circ_0084927 group (p<0.05). Optical density value,
scratch healing rate, clone numbers as well as invasion numbers in the DDP+microRNA-634 group were
significantly reduced relative to the DDP+microRNA-NC group (p<0.05). Circ_0084927 directly bound
to microRNA-634. Relative to the DDP+si-circ_0084927+anti-microRNA-NC group, optical density
value, scratch healing rate, clone numbers as well as invasion numbers in the DDP+si-circ_0084927+anti-
microRNA-634 group were increased (p<0.05). Interference with circ_0084927 reduced the cisplatin
resistance of lung cancer cells by promoting microRNA-634.
Click on an image to view it in the image viewer
There are no comments for this item.